objective(s) this study planned to assess the value of pcr is6110 assay in tissue specimens of needle pleural biopsy in patients suspicious to pleural tuberculosis. materials and methods sixty eight patients with lymphocytic exudative pleural effusion underwent pleural biopsy. tissue samples were sent for pathologic examination and pcr is6110 assay. the results of pcr reported as positive/ nega...

three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

purpose : the evaluation of the presence of herpes simplex virus (hsv) in corneal scars by real-time polymerase chain reaction (pcr) and comparison of the results with histopathologic findings and clinical diagnoses methods : eighty-seven corneal scar samples obtained after penetrating keratoplasty (pk), were selected after reviewing the records from 2006 to 2010. nucleic acid was extracted fro...

background: the study attempts to assess the relationship between chimerism analysis using polymerase chain reaction of short tandem repeat (str) and the incidence of chronic graft versus host disease (gvhd) as well as survival. methods: the retrospective cohort included all patients who received allo-hsct between 2005 to 2013. data collected by day +100 were reviewed in terms of the incidence ...

conclusions in this study b19-parvovirus infection were detected in patients with hematologic disorders in comparison with control subjects. screening of patients with a high risk of parvovirus b19 infection can considerably reduce the incidence and prevalence of b19 infection. results the parvovirus b19-specific nucleotide sequence was detected in 6 patients (20%). none of the samples obtained...

background bacterial resistance to antibiotics has become a major source of concern for public health. pseudomonas aeruginosa strains are important opportunistic pathogens. these bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics. objectives the present study was performed to evaluate the frequency of kpc in p. aeruginosa isolated from clinical samples of...

objectives the objective of this study was to develop the primers for single and/or multiplex pcr amplification assays for simultaneous identification of class a, class b, and class d carbapenem hydrolyzing β-lactamases in enterobacteriaceae and then to evaluate their efficiency. conclusions we have reported here a reliable method, composed of single and multiplex pcr assays, for screening all ...

conclusions the determination of serotype and surface proteins of gbs strains distribution would ‎be ‎relevant ‎for the future possible formulation of a gbs vaccine. patients and methods in this cross-sectional study, a total of 100 gbs strains isolated from 237 pregnant and 413 non-pregnant women were investigated for molecular capsular serotypes and surface protein genes using the multiplex p...

conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

background the occult hepatitis b infection (obi) is defined as the presence of hepatitis b virus (hbv) dna in the sera or in the liver biopsy and the absence of hepatitis b surface antigen (hbsag) by serological test. objectives the current study aimed to evaluate the occult hbv infection by polymerase chain reaction (pcr) and determine hbv genotyping among the patients with abnormal alanine t...