OBJECTIVE To evaluate the effect of low dose methotrexate alone or in combination with glucocorticoid treatment on titanium implant osseointegration. METHODS Groups of 6-8 adult New Zealand White rabbits were treated for 18 weeks with saline (control), methotrexate, glucocorticoid, or methotrexate plus glucocorticoid. The animals received a titanium implant in the tibia at week 6. Lumbar spin...

this study redescribes an arthroscopic bridge technique for repair of avulsion of the posterior cruciate ligament. the procedure is performed step-to-step. in this technique we created two bone tunnels in the anterior aspect of the tibia and inferior medial of the tibia tuberosity and then create suture fixation of the fragment using the suture bridge. the arthroscopic bridge technique was used...

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer were specifically developed to transfect nucleic acids into mammalian cells and difficult-to-transfect cells, such as primary and stem cells.This video demonstrates how to es...

Two experiments were conducted to compare a sample of calcium citrate-malate (CC-M) with a sample of commercial-grade limestone in starting broiler chick diets. In the first experiment, with 0.7 or 0.9% calcium from limestone or CC-M, no differences in bone development (dry fat-free tibia, tibia weight, tibia ash, or tibia calcium) were observed due to calcium source. However, chicks fed the di...

Reporting two cases one of centimetres breadth and the other four exposed tibia bone devoid periosteum due to post debridement involving elevator remove infective tissue which was successfully covered by a simple procedure with MatriDerm® split skin graft in single stage. Tibia not drilled sprout granulation from marrow create granulated vascularised bed for second-stage grafting. It avoided lo...

We report a technique of implantation of the ipsilateral vascularized fibula with pasteurized recycled bone after excision of tibia sarcoma in two cases. Plate and screws were used for osteosynthesis of the tibia or talus, vascularized fibula, and pasteurized bone. Microsurgery is not necessary for this reconstruction technique. Two patients who underwent this technique have obtained good funct...

Nutrition is a primary modifiable determinant of chronic noncommunicable disease, including osteoporosis. An etiology of osteoporosis is the stimulation of bone-resorbing osteoclasts by reactive oxygen species (ROS). Dietary polyphenols and probiotics demonstrate protective effects on bone that are associated with reduced ROS formation and suppressed osteoclast activity. This study tested the e...

BACKGROUND Measurement of bone mineral density by dual x-ray absorptiometry combined with clinical risk factors is currently the gold standard in diagnosing osteoporosis. Advanced imaging has shown that older patients with fragility fractures have poor bone microarchitecture, often independent of low bone mineral density. We hypothesized that premenopausal women with a fracture of the distal en...

An experiment was conducted to investigate the effect of dietary Ca level on the efficacy of phytase. A total of 288 male Ross × Ross 708 broilers with initial and final BW of 37 and 705 g, respectively, were used in brooder batteries from 0 to 21 d posthatch. Each treatment had 8 replications with 6 broilers/replicate pen. All diets were corn-soybean meal based and formulated to contain 1.26% ...

The ability of an interlocking screw fixation technique to minimize bone loss related to stress shielding in the tibia was investigated and compared to the abilities of cement and press-fit fixation. Full bony ingrowth has been associated with greater stress shielding than partial ingrowth; therefore, the effect of intimate bonding of the stem to bone on subsequent bone loss was also studied. A...