The Ras oncogene products regulate the expression of genes in transformed cells, and members of the Ets family of transcription factors have been implicated in this process. To determine which Ets factors are the targets of Ras signaling pathways, the abilities of several Ets factors to activate Ras-responsive enhancer (RRE) reporters in the presence of oncogenic Ras were examined. In transient...

The yeast Snf1 kinase and its metazoan orthologues, the AMP-activated protein kinases, are activated in response to nutrient limitation. Activation requires the phosphorylation of a conserved threonine residue in the activation loop of the catalytic subunit. A phosphopeptide antibody was generated that specifically recognizes Snf1 protein that is phosphorylated in its activation loop on threoni...

This study was conducted to find the effect of methionine and threonine supplementations higher than the NRC recommendation on growth performance and white blood cell differentiation of broiler chickens challenged with infectious bursal disease. A total of 450 day-old male broiler chicks were assigned to nine groups. Chickens were fed by three graded levels of DLmethionine [NRC (M1), 2 times NR...

A threonine-producing strain of Serratia marcescens Sr41 was constructed according to the following process. Thr- strain E-60 was derived from strain HNr59 having constitutive levels of threonine-sensitive aspartokinase and homoserine dehydrogenase. Thr+ transductant T-570 was constructed from strain E-60 and phage grown on strain HNr21 having feedback-resistant threonine-sensitive aspartokinas...

We have tested the hypothesis that the mechanism of platelet-derived growth factor (PDGF) and phorbol diester action to decrease the apparent affinity of the epidermal growth factor (EGF) receptor is the phosphorylation of the EGF receptor at the Ca2+/phospholipid-dependent protein kinase (protein kinase C) phosphorylation site, threonine 654. Protein kinase C-deficient cells were prepared by p...

The Impact of Supplemental L-Threonine in Laying Hen Diets on Egg Component Yield, Composition, and Functionality. (August 2005) Paige Reynolds Niemeyer, B.S., Texas A&M University; M.S., Texas A&M University Chair of Advisory Committee: Dr. John Carey The impacts of supplemental L-threonine in laying hen diets were evaluated. Over three experiments, control hens were fed a corn-soybean commerc...

Threonine dehydrase (deaminase), which catalyzes the conversion of I,-threonine to a-ketobutyrate and ammonia, has been observed in crude extracts prepared from a number of different organisms (1-13). Frequently these same extracts also act on L-serine to form pyruvate and ammonia. Table I summarizes the available data on the serine and threonine dehydrasel activities in crude extracts from var...