An interference between a thermosensitive (ts) mutant and the wild-type (wt) of tick-borne encephalitis (TBE) virus in Ixodes ricinus L. and Rhipicephalus appendiculatus (Neumann) ticks is reported. I. ricinus females were dually infected by a parenteral inoculation of ts and wt strains at 10-day interval. Interference was demonstrated by the lowered ability of wt virus to replicate in ticks pr...

To prepare lomustine-iohexol thermosensetive compound liposomes, and evaluate the properties and in vitro release behaviors of two drugs enveloped. Lomustine-iohexol thermosensetive compound liposomes were prepared by reverse evaporation method, HPLC assays for determination of lomustine and iohexol in vitro were developed, and their release property was studied by using dislysis method. The me...

Thermosensitive hydrogels have increasingly received considerable attention for local drug delivery based on many advantages. However, burst release of drugs is becoming a critical challenge when the hydrogels are employed. Microspheres- (MS-) loaded thermosensitive hydrogels were thus fabricated to address this limitation. Employing an orthogonal design, the spray-dried operations of tenofovir...

Two mutants (NT02 and NT17), each producing a thermosensitive neutral protease, were isolated from Bacillus subtilis NP58, a transformant which acquired the property of hyperproduction of neutral protease from Bacillus natto IAM 1212. The neutral proteases produced by these two mutants were partially purified and enzymologically characterized. The two mutant neutral proteases displayed increase...