نتایج جستجو برای: thermophilic proteins

تعداد نتایج: 561306  

2012
Eva Hammann Andrea Zappe Stefanie Keis Stefan Ernst Doreen Matthies Thomas Meier Gregory M. Cook Michael Boersch

Thermophilic enzymes can operate at higher temperatures but show reduced activities at room temperature. They are in general more stable during preparation and, accordingly, are considered to be more rigid in structure. Crystallization is often easier compared to proteins from bacteria growing at ambient temperatures, especially for membrane proteins. The ATP-producing enzyme FoF1-ATP synthase ...

2017
Fei Leng Lu-Yun Wu Chang Lu Xian-Ming Pan

Protein thermostability has received growing attention in recent years. Little is known about the determinants of thermal resistance in individual protein families. However, it is known that the mechanism is family-dependent and not identical for all proteins. We present a multivariate statistical analysis to find the determinants of thermostability in one protein family, the serine hydroxymeth...

Journal: :The Biochemical journal 2004
Annamaria Guagliardi Lucia Mancusi Mosè Rossi

In eukaryotic cells and in Escherichia coli, reversion of protein aggregation is mediated by the network of chaperones belonging to Hsp70 and Hsp100 families [Weibezahn, Bukau and Mogk (2004) Microb. Cell Fact. 3, 1-12]. The thermophilic prokaryotes of the archaea domain lack homologues of these chaperone families, and the mechanisms they use to rescue aggregated proteins are unknown [Macario, ...

2017
Judit Ribera Mónica Estupiñán Alba Fuentes Amanda Fillat Josefina Martínez Pilar Diaz

A search for extremophile enzymes from ancient volcanic soils in El Hierro Island (Canary Islands, Spain) allowed isolation of a microbial sporulated strain collection from which several enzymatic activities were tested. Isolates were obtained after sample cultivation under several conditions of nutrient contents and temperature. Among the bacterial isolates, supernatants from the strain design...

2015
Tarik Aanniz Mouna Ouadghiri Marouane Melloul Jean Swings Elmostafa Elfahime Jamal Ibijbijen Mohamed Ismaili Mohamed Amar

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the g...

2015
Elise A. Rooney Kenneth T. Rowe Anna Guseva Marcel Huntemann James K. Han Amy Chen Nikos C. Kyrpides Konstantinos Mavromatis Victor M. Markowitz Krishna Palaniappan Natalia Ivanova Amrita Pati Konstantinos Liolios Henrik P. Nordberg Michael N. Cantor Susan X. Hua Nicole Shapiro Tanja Woyke Lee R. Lynd Javier A. Izquierdo

Clostridium clariflavum strain 4-2a, a novel strain isolated from a thermophilic biocompost pile, has demonstrated an extensive capability to utilize both cellulose and hemicellulose under thermophilic anaerobic conditions. Here, we report the draft genome of this strain.

Journal: :Applied and environmental microbiology 1990
R Beaudet C Gagnon J G Bisaillon M Ishaque

A thermophilic strain (D2) identified as a Bacillus sp. was isolated from an aerobic digestor of swine waste after several months of operation at 55 degrees C. Aerobic thermophilic batch treatment of swine waste inoculated with strain D2 was studied in a 4-liter fixed-bed reactor. Stabilization of the waste was achieved in less than 30 h when the original chemical oxygen demand (COD) was betwee...

2016
Wei Sun Xun Qian Jie Gu Xiao-Juan Wang Man-Li Duan

Animal manure comprises an important reservoir for antibiotic resistance genes (ARGs), but the variation in ARGs during anaerobic digestion at various temperatures and its underlying mechanism remain unclear. Thus, we performed anaerobic digestion using dairy manure at three temperature levels (moderate: 20 °C, mesophilic: 35 °C, and thermophilic: 55 °C), to analyze the dynamics of ARGs and bac...

2015
Dara S. M. Ghasimi Yu Tao Merle de Kreuk Marcel H. Zandvoort Jules B. van Lier

BACKGROUND In this research, the feasibility of, and population dynamics in, one-step anaerobic sequencing batch reactor systems treating the fine sieved fraction (FSF) from raw municipal wastewater was studied under thermophilic (55 °C) and mesophilic (35 °C) conditions. FSF was sequestered from raw municipal wastewater, in the Netherlands, using a rotating belt filter (mesh size 350 micron). ...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد علوم دارویی - پژوهشکده علوم 1392

purpose a metabolic abnormality such as obesity is a major obstacle in the maintenance of the human health system and causes various chronic diseases including type 2 diabetes, hypertension, cardiovascular diseases, as well as various cancers. this study was designed to summarize the recent scientific knowledge regarding the anti-obesity role of curcumin (diferuloylmethane), which is isolated f...

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