Here we report a thermodynamic analysis of biomolecular interactions using MicroScale Thermophoresis (MST). Using DNA hybridization with fluorescently labeled oligonucleotides as a well-characterized model system, we monitor the shift in the dissociation (Kd) constant of the interaction over a range of temperatures and calculate the free enthalpy (ΔH) and entropy (ΔS) for the hybridization reac...
