Formazan-based colorimetric cytotoxicity assays, such as the MTT assay, are typically used to assess cell viability with only metabolically active cells reducing tetrazolium salts into the formazans, which is then quantified by absorbance. Fluorescence offers several advantages compared to colorimetric assays and would enable techniques such as flow cytometry and confocal microscopy to be used ...

Two methods of detecting early inapparent myocardial infarcts have been studied and their value in diagnostic practice compared. The better method proved to be the determination of the potassium to sodium ratio (ionic ratio) which falls in infarcted tissue within minutes of the onset of anoxia. The second method was nitro blue tetrazolium staining of gross sections of myocardium which revealed ...

Tetrazolium dyes have often been used as electron acceptors in measurements of cell proliferation rates (Berridge and Tan, 1993; Ishiyama, et al., 1993; Berridge, et al., 1996), in histochemical procedures (Zamudio, et al., 1969; May, et al., 1995b; Demehin, et al., 2001), and for assaying redox enzymes (Berridge and Tan, 1993; Mittler and Zilinskas, 1993; Debnam and Shearer, 1997; Ye, et al., ...

Bacterial β-galactosidase is one of the most widely used reporter genes in experiments involving transgenic and knockout animals. In this review we discuss the current histochemical methods and available reagents to detect β-galactosidase activity. Different substrates are available, but the most commonly used is X-gal in combination with potassium ferri- and ferro-cyanide. The reaction produce...

The method of evaluating cold injury in woody plants by the use of triphenyl tetrazolium chloride has been refined to eliminate bias associated with visual differentiation between varying degrees of tetrazolium reduction and to predict tissue survival at a later date. An advantage of the method described here is that a small amount of tissue (50-100 mg) is required; this, therefore, allows for ...

ABSTRACT An essential factor for the formation of a native orchid seed bank is identification viability stored seeds. This study aimed to determine methodology optimizing tetrazolium test, in seeds two Orchidaceae medicinal species Brazilian Savanna. Seeds Miltonia flavescens Lindl. and Schomburgkia crispa were submitted three pre-conditioning conditions: no soaking (control), sucrose solution ...