Confluent monolayers of the human hepatoblastoma-derived cell line, Hep G2, were incubated in serum-free medium. Conditioned medium was ultracentrifugally separated into d less than 1.063 g/ml and d 1.063-1.20 g/ml fractions since very little VLDL was observed. The d less than 1.063 g/ml fraction was examined by electron microscopy; it contained particles of 24.5 +/- 2.3 nm diameter, similar in...

bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. the aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...

BACKGROUND The microbiomes of humans are associated with liver and lung inflammation. We identified and verified alterations of the oropharyngeal microbiome and assessed their association with cirrhosis and pneumonia. METHODS Study components were as follows: (1) determination of the temporal stability of the oropharyngeal microbiome; (2) identification of oropharyngeal microbial variation in...

Granular biomass from a laboratory-scale anaerobic bioreactor trial was analysed to identify changes in microbial community structure and function in response to temperature and trichloroethylene (TCE). Two bioreactors were operated at 37°C, while two were operated at 15°C. At the time of sampling, one of each temperature pair of bioreactors was exposed to process failure-inducing concentration...

Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low content of GC pairs. This paper describes how treatment of template DNA with sodium bisulphite drastic...

A strain of Fusobacterium nucleatum which produced high levels of beta-lactamase was isolated. The specific activity of the unpurified beta-lactamase was 7.8 U/mg of protein. By Sephacryl S-300 and S-200 column passage and chromatofocusing, the enzyme was purified 450-fold. Sodium dodecyl sulfate-gradient gel electrophoresis revealed a single band. The enzyme hydrolyzed phenoxymethylpenicillin,...