Translational control of polypeptide synthesis was measured with ribosomal preparations obtained from brain tissue from mice of various ages. Ribosomal cell-free reaction mixtures were employed to measure the relative activities of the monomeric ribonucleoprotein particles during early neural development. Cell-free protein synthesis was markedly reduced during the first few days after birth and...

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The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Simil...

a series of tri- and tetra- substituted imidazoles were synthesized from benzyl, aldehyde and ammonium acetate in the presence of zeolite as an ecofriendly reusable catalyst under microwave irradiation in the absence of solvent. the yields are high to excellent and the use of microwave irradiation reduces reaction times to few minute.

Single-stranded M13mp18 phage DNA was methylated with dimethylsulfate (DMS), and further treated with alkali to ring-open N7-methylguanine residues and yield 2-6-diamino-4-hydroxy-5N-methylformamidopyrimidine (Fapy) residues. Nucleotide incorporation during in vitro DNA synthesis on methylated template using E. coli DNA polymerase Klenow fragment (Kf polymerase) was reduced compared to the unme...

The genome of poliovirus consists of a single strand of RNA approximately 7.5 kb long. Analysis of the sequences around 40 unique recombination sites reveals several features that differ significantly from those expected of randomly located sites. These features, which include a broad zone of elevated homology on the 3' side of the cross-over, support the theory that RNA recombination occurs by...

A full-length cDNA copy of the phosphoprotein (NS) mRNA of vesicular stomatitis virus (New Jersey serotype) was inserted into pGEM4 vector downstream of the promoter for bacteriophage SP6 RNA polymerase. Transcription of the cDNA in vitro resulted in the synthesis of NS mRNA, which was subsequently translated into NS protein in a cell-free rabbit reticulocyte system. The biological activity of ...

Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that repairs the discontinuities that form in the DNA synthesized from damaged templates. Although tr...

Herein, we report the synthesis of metallic molybdenum microspheres and hierarchical MoS2 nanostructures by facile template-free solvothermal and hydrothermal approach, respectively. The morphological transition of the Mo microspheres to hierarchical MoS2 nanoflower architectures is observed to be accomplished with change in solvent from ethylenediamine to water. The resultant marigold flower-l...

this study describes the synthesis of molecularly imprinted polymers (mips) by using an anti-che ops, namely dursban, as a template. non-covalent bulk polymerization was successfully applied to synthesis different imprinted and non-imprinted polymers with maa, mma, aa, and 4-vpy as monomer in selected porogens (chloroform, toluene, and acetonitrile). in order to evaluate the template binding of...