In sample-stacking techniques, the detection limit cannot be improved by simply increasing the length of the sample solution, because the individual electrophoretic parameters must be optimized. In an attempt to increase the amount of sample injected, as well as to focus them onto a small zone, two novel methods are proposed. One of these employs an "ultra-high conductivity zone", which was ins...

Shock is often under-reported in children attending hospitals in developing countries. Readily obtainable features of shock (capillary refill time, temperature gradient, pulse volume, and signs of dehydration) are widely used to help prioritise management in the emergency assessment of critically ill or injured children. However, data are lacking on their validity, including, importantly, repro...

We present a generic way of forming various complex polymeric microstructures using physical self-organization and capillary dynamics. A simple lithographic tool called capillary force lithography is utilized for this purpose, in which the pattern formation is driven by capillary force, not involving any external force or modification. In this method, a patterned polydimethylsiloxane mold is pl...

A fast SPE-GC-ECD method for the analysis of PCBs in waste oil was developed. A complete profi le was obtained following SPE with a 12 minute GC run-time using a modular accelerated column heater (MACH). Full automation of the sample preparation and analysis (except sample weigh-in) enables a daily throughput of 100 samples. A wide range of concentrations can be determined using a dedicated col...

Here we report the use of capillary isoelectric focusing under native conditions for the separation of protein complex isoforms and subcomplexes. Using biologically relevant HIS-tag and FLAG-tag purified protein complexes, we demonstrate the separations of protein complex isoforms of the mammalian target of rapamycin complex (mTORC1 and 2) and the subcomplexes and different phosphorylation stat...

We have previously demonstrated the potential usefulness of capillary columns coated with a high temperature polarizable phenylmethylsilicone liquid phase in plasma lipid profiling (Kuksis, A., J.J. Myher, and P. Sandra. 1990. J. Chromatogr. 500: 427-441). The present study reports improved operating conditions along with a practical application to the analysis of a series of human plasma sampl...

A stereospecific capillary zone electrophoresis (CZE) method was developed for the determination of verapamil (VER) and its main metabolite norverapamil (NOR) in human plasma. Optimal temperature, cyclodextrin selectors (CDs), pH of background electrolyte (BGE) and voltage were established to obtain complete separation of the chiral analytes and clopidogrel internal standard (I.S.) during one a...