conclusions despite of high resistance of p. aeruginosa, the resistance rate of p. aeruginosa strains isolated from children in the current study was not high. then accurate prescription of antibiotics can decrease the speed of resistance creation. background the first report of pseudomonas aeruginosa (p. aeruginosa) carbapenem resistant strains, and especially the metallo-beta-lactam (mbl) pro...

conclusions regarding the importance of metallo-beta-lactamase (mbl) produced by pseudomonas spp. isolated from hospitals, quick identification of clinical samples should be considered as an important and basic step to treat and control infections caused by pseudomonas spp. results strains with mic > 4 µg/ml were investigated by phenotypic and genotypic methods. in the current study the rate of...

The clinical strain Escherichia coli TO799 was resistant to penicillin-clavulanate combinations and ceftazi-dime and was not reproducibly detected as an extended-spectrum ␤-lactamase (ESBL) according to the standards of the Clinical Laboratory Standards Institute (CLSI; formerly NCCLS) and the national guidelines of the French Society for Microbiology (Comité de l'Antibiogramme de la Société Fr...

We report an outbreak of Enterobacter aerogenes in an intensive care unit (ICU) and two medicine departments that produced the extended-spectrum beta-lactamase TEM-24, which was difficult to detect by disk agar diffusion. The strains were compared by DNA restriction fragment length polymorphism after pulsed-field gel electrophoresis following cleavage with XbaI. This typing method indicated tha...

Phylogenetic analyses of molecular data require a quantitative model for how sequences evolve. Traditionally, the details of the site-specific selection that governs sequence evolution are not known a priori, making it challenging to create evolutionary models that adequately capture the heterogeneity of selection at different sites. However, recent advances in high-throughput experiments have ...

A ceftazidime-resistant Salmonella enterica subspecies enterica serotype Othmarschen strain, harboring the plasmid-mediated new extended-spectrum beta-lactamase TEM-27, was involved in a nosocomial outbreak (8 patients) at the Pediatric Cardiology Department of the Ramón y Cajal Hospital in Madrid. Genomic DNA polymorphism analysis, using an arbitrarily primed polymerase chain reaction, demonst...

The clinical outcome in patients treated with ampicillin-sulbactam may not always be predictable by disc susceptibility testing or with the MIC as determined with a constant level (4 micrograms/ml) of the beta-lactamase inhibitor (MIC1). The enzyme activities (EA) and the MICs estimated at a constant ratio of ampicillin to sulbactam of 2:1 (MIC2) for 15 TEM-1 beta-lactamase-producing strains of...

An isolate of Aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. Transfer of the plasmid to Escherichia coli K12 resulted in the expression of a TEM-like beta-lactamase that was not expressed in parental A. caviae. The bla gene sequence was detectable by DNA hybridization and PCR amplification of the plasmid when extracted from parental A. caviae or from E. coli K12 transconjugan...