Zhixian Zhou,1,* Rongying Jin,1 Gyula Eres,1 David Mandrus,1 Victor Barzykin,2 Pedro Schlottmann,3 Yew-San Hor,4 Zhili Xiao,4,5 and John F. Mitchell4 1Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA 2Department of Physics and Astronomy, University of Tennessee, Knoxville, Tennessee 37996, USA 3Department of Physics, Florida State Univers...

to treat melanomas, lung, head, and neck cancers. Ornamental yews have been identified as a potential renewable source of taxol and related taxanes. Commercial nurseries were surveyed during Summer and Fall 1991 as a basis for estimating populations of Taxus cultivars currently growing in the United States. Clippings of selected cultivars were sampled from nursery fields in Ohio Production & Ma...

A new full-length acyltransferase cDNA was obtained from Taxus chinensis by homology-based cloning strategy. The cDNA has an open-reading frame of 1,275 nucleotides, which encodes 425 amino acids with a calculated molecular weight of 47,241 Da and an estimated pI value of 5.93. The deduced amino acid sequence resembles the sequences of other cloned acyltransferases (56-61% identity; 71-75% simi...

We have investigated the Taxus cuspidata pollinosis that has never been studied before. We applied immunoblotting method in order to detect specific IgE antibody against T. cuspidata using its pollen itself. Out of 18 patients with seasonal allergic rhinitis suffering mainly in April and May, 5 patients were sensitized with this pollen. Furthermore, we were able to diagnose one case as T. cuspi...

The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-4(1) that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics...

Suspension cultures of Taxus canadensis were elicited with methyl jasmonate (MJ) under defined headspace ethylene concentrations. Kinetic studies of growth, nutrient consumption, pH variation, and paclitaxel accumulation were conducted in batch cultures and semicontinuous culture with total cell recycle. A dramatic increase of paclitaxel was obtained when the cultures were elicited with 100 mic...