Simultaneous saccharification and fermentation (SSF) experiments were performed at selected temperatures (37, 41, and 43 degrees C) to obtain comprehensive material balance and performance data for several promising strains of thermotolerant yeast. Parameters measured were ethanol concentration, yeast cell density, and residual sugar and cellulose concentrations. The three yeasts Saccharomyces ...

Insecticidal Bacillus thuringiensis (Bt) 8-endotoxins are cytolytic to a range of insect cell lines in vitro. Addition of Bt var. aizawai or var. israelensis toxins to Mamestra brassicae (cabbage moth) cells in vitro increased intracellular cyclic AMP, which was paralleled by activation of adenylate cyclase in isolated membranes. Var. kurstaki toxin, which does not lyse M. brassicae cells, had ...

The effects of plant competition for light on the emission of plant volatile organic compounds (VOCs) were studied by investigating how different light qualities that occur in dense vegetation affect the emission of constitutive and methyl-jasmonate-induced VOCs. Arabidopsis thaliana Columbia (Col-0) plants and Pieris brassicae caterpillars were used as a biological system to study the effects ...

Pyrenopeziza brassicae, cause of light leaf spot (LLS), is an important pathogen oilseed rape and vegetable brassicas has a wide geographic distribution. Exploitation host resistance remains the most sustainable economically viable solution for disease management. This study evaluated 18 cultivars or breeding lines against P. brassicae in glasshouse experiments. Selected cultivars/lines were in...

Herbivore insects are suitable model organisms for studying how plant odor information is encoded in olfactory receptor neurons (RNs). By the use of gas chromatography linked to electrophysiological recordings from single RNs, screening for sensitivity to naturally produced plant odorants is possible in order to determine the molecular receptive ranges of the neurons. Using this method, we have...

The population dynamics of major brassica pests and occurrence of their natural enemies were monitored weekly for a period of 9 months on cabbage (var. Copenhagen Market) at a research station plot (no pesticides applied) and a farmer’s field (weekly application of Permethrin 10 EC or Dimethoate 40 EC) in Central Kenya. Pest infestations occurred within 14 days after the seedlings were transpla...