نتایج جستجو برای: suppression subtractive hybridization

تعداد نتایج: 160708  

Journal: :Journal of bacteriology 2004
David DeShazer

Burkholderia pseudomallei is the etiologic agent of the disease melioidosis and is a category B biological threat agent. The genomic sequence of B. pseudomallei K96243 was recently determined, but little is known about the overall genetic diversity of this species. Suppression subtractive hybridization was employed to assess the genetic variability between two distinct clinical isolates of B. p...

Journal: :DNA and cell biology 2002
Jaerang Rho Curtis R Altmann Nicholas D Socci Lubomir Merkov Nacksung Kim Hongseob So Okbok Lee Masamichi Takami Ali H Brivanlou Yongwon Choi

Bone homeostasis is maintained by the balanced action of bone-forming osteoblasts and bone-resorbing osteoclasts. Multinucleated, mature osteoclasts develop from hematopoietic stem cells via the monocyte-macrophage lineage, which also give rise to macrophages and dendritic cells. Despite their distinct physiologic roles in bone and the immune system, these cell types share many molecular and bi...

Journal: :Applied and environmental microbiology 2006
Agnès Cimerman Guillaume Arnaud Xavier Foissac

Phytoplasmas are unculturable bacterial plant pathogens transmitted by phloem-feeding hemipteran insects. DNA of phytoplasmas is difficult to purify because of their exclusive phloem location and low abundance in plants. To overcome this constraint, suppression subtractive hybridization (SSH) was modified and used to selectively amplify DNA of the stolbur phytoplasma infecting a periwinkle plan...

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