Rat liver nuclei, after preliminary isolation in 2.2 molar sucrose solution, were separated into density classes by centrifugation at 95,000 g for 45 to 85 minutes in a sucrose density gradient (density range, 1.28 to 1.33). Nuclei from normal liver separated into three bands with average DNA phosphorus content per nucleus of 0.67, 0.84, and 0.93 picogram for top, middle, and bottom bands, resp...

Trehalose 6-phosphate (Tre6P), the intermediate of trehalose biosynthesis, has a profound influence on plant metabolism, growth, and development. It has been proposed that Tre6P acts as a signal of sugar availability and is possibly specific for sucrose status. Short-term sugar-feeding experiments were carried out with carbon-starved Arabidopsis thaliana seedlings grown in axenic shaking liquid...

Changes in total starch and reducing sugar content in five sweetpotato varieties were investigated weekly during root development and following subjection of the roots to different postharvest handling and storage conditions. Freshly harvested (noncured) roots and cured roots (spread under the sun for 4 days at 29-31°C and 63-65% relative humidity [RH]) were separately stored at ambient conditi...

In sugar beet (Beta vulgaris L.), taproot weight and sucrose content are the important determinants of yield and quality. However, high yield and low sucrose content are two tightly bound agronomic traits. The advances in next-generation sequencing technology and the publication of sugar beet genome have provided a method for the study of molecular mechanism underlying the regulation of these t...

Soluble sugar and malic acid contents in mature fruits of 364 apple accessions were quantified using high-performance liquid chromatography (HPLC). Fructose and sucrose represented the major components of soluble sugars in cultivated fruits, whilst fructose and glucose were the major items of sugars in wild fruits. Wild fruits were significantly more acidic than cultivated fruits, whilst the av...

Fusarium wilt diseases caused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Biocontrol agents such asTrichoderma viride , Trichoderma harzianum and Pseudomonas fluorescens are applied to control Fusarium wilt of Arachis hypogaea. Experiments were conducted on the effect of culture filtrates of T.viride (1%), T.harzianum (1.5%), and P. fluorescens (2%) on biochemica...

Daily fat and sugar intake has increased in Japan, while total energy decreased. However, the number of type 2 diabetes mellitus patients increased, this often causes renal injury characterized by autophagic vacuoles. Although many studies with comparisons high or versus a normal macronutrient balanced diet have been reported, there are few that equalized calorie body weights. In current study,...

PURPOSE The purpose of this study was to evaluate the release of recombinant human growth hormone (rhGH) from a novel non-polymeric sustained release system, SABER. METHODS The SABER system consists of sucrose acetate isobutryate, a solvent and a polymeric release modifier. Spray dried formulations of zinc complexed rhGH in sodium bicarbonate containing sucrose and polysorbate 20 were homogen...

Hairy roots infected with Agrobacterium rhizogenes were induced from roots, stems, and leaves of Korean ginseng (Panax ginseng C.A. Meyer) on half strength MS medium supplemented with 300 mg·L Cefotaxim sodium. DNA extraction was carried out and PCR results confirmed that the hairy roots induced by A. rhizogenes KCTC 2703 have the rol C gene in their T-DNA. Selected root lines were propagated i...

The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 degrees C for 3 weeks and then transferred to a constant 5 degrees C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14- to 20-fold over ...