BACKGROUND Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process. OBJECTIVE In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after ...

We studied functional disturbances following left middle cerebral artery occlusion in rats. Neuronal function was evaluated by [14C]2-deoxyglucose autoradiography 1 day after occlusion. We analyzed the mechanisms of change in glucose utilization outside the infarct using Fink-Heimer silver impregnation, axonal transport of wheat germ agglutinin-conjugated-horseradish peroxidase, and succinate d...

The photosensitizing activity of hematoporphyrin derivative (HPD) was investigated by studying selected enzymes localized to mitochondria and cytosol of R3230AC mammary adenocarcinomas. Experiments in vitro demonstrated that mitochondrial succinate dehydrogenase was inhibited in a drug dose- and light exposure time-related manner; at 7.0 micrograms of HPD per ml or higher, enzyme activity was i...

Canavan disease (CD) is an autosomal recessive disorder caused by aspartoacylase deficiency leading to accumulation of N-acetylaspartic acid and spongy degeneration of the brain. The mouse model for CD showed low levels of glutamate and gamma-aminobutyric acid (GABA) in the brain. Whether the low levels of glutamate and GABA observed in the CD mouse brain lead to abnormal production of glutamat...

418 Structure and function of flavocytochrome c3, the soluble fumarate reductase from Shewanella NCI M 6400 G. A. Reid*', E. H. J. Gordon*', A. E. Hill*, M. Dohertyt, K. Turnert, R. Halt* and S. K. Chapmant *Institute of Cell and Molecular Biology and tDepartment of Chemistry, University of Edinburgh, Mayfield Road, Edinburgh EH9 3]R, Scotland, U.K., and SZeneca Life Science Molecules, Belasis ...

a preliminary communication (Jackson & Lightbown, 1956) we have reported the inhibitory effect of heptyl N-oxide on the dihydrocozymaseoxidase activities of heart-muscle succinic oxidase preparations and of certain cell-free preparations of Staphylococcus aureus, Eacherichia coli, Proteus vulgariw and Bacillus pumilu&. The present paper includes a more detailed account of this work, together wi...

Phages capable of transducing succinate dehydrogenase mutants (sdh) of Escherichia coli were isolated from pools of artificially constructed recombinant lambda phages using a selective casein digest medium. These phages produced characteristically dense turbid plaques, and as prophages they increased the aerobic growth efficiencies of sdh mutants on complex media but were unable to promote grow...

Cell suspensions of Campylobacter fetus subsp. intestinalis grown microaerophilically in complex media consumed oxygen in the presence of formate, succinate, and DL-lactate, and membranes had the corresponding dehydrogenase activities. The cells and membranes also had ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine oxidase activity which was cyanide sensitive. The fumarate reductase activity...

Succinate dehydrogenase (Complex II or succinate-ubiquinone oxidoreductase) is the smallest complex in the respiratory chain and transfers the electrons derived from succinate directly to the ubiquinone pool. Succinate dehydrogenase inhibitor (SDHI) fungicides specifically inhibit fungal respiration by blocking the ubiquinone-binding sites in the mitochondrial complex II and play an important r...

Corynebacterium glutamicum lacking the succinate dehydrogenase complex can produce succinate aerobically with acetate representing the major byproduct. Efforts to increase succinate production involved deletion of acetate formation pathways and overexpression of anaplerotic pathways, but acetate formation could not be completely eliminated. To address this issue, we constructed a pathway for re...