the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...

streptococcus agalactiae , s. dysgalactiae and s. uberis are gram-positive bacterial pathogens that affect cows in dairy herds. these are considered as the major causes of economic losses of dairy producers without a control program. to reveal the role of these agents in inducing subclinical mastitis in dairy cattle in ahvaz, 100 cmt-positive milk samples and 20 cmt-negative milk samples were c...

background and objectives: streptococcus pneumoniae is the most common cause of invasive infections among both young children and elderly people. common serotypes causing invasive diseases and the emergence of carriers of streptococcus pneumoniae in iran is not yet known. past-vaccine surveillance studies of serotype prevalence patterns in iran are necessary to monitor the epidemiology of strep...

The novel, Gram-stain-positive, ovoid, lactic acid bacterial isolates LMG 27205, LMG 27206, LMG 27207(T) and MRI-F 18 were obtained from throat samples of healthy humans. 16S rRNA gene sequence analyses indicated that these isolates belong to the genus Streptococcus, specifically the Streptococcus mitis group, with Streptococcus australis and Streptococcus mitis as the nearest neighbours (99.45...

This article discusses Streptococcus salivarius meningitis in a 39-year old woman. She was admitted to the hospital for general fatigue, headache, and fever. Cerebrospinal fluid analysis revealed leukocytosis and cultures were positive for Streptococcus salivarius. Streptococcus salivarius meningitis is rare and has been documented to be associated with procedures involving the spinal column su...

OBJECTIVES Ciprofloxacin is the most frequently used member of the fluoroquinolones during initial eradication therapy of Pseudomonas aeruginosa, as well as during acute pulmonary exacerbations. However, its long-term effect on the susceptibility of the commensal flora within the cystic fibrosis (CF) airways has not yet been examined. The aim of this study was therefore to examine the consequen...

Mastitis, a severe infectious disease among dairy cattle, is still a challenge for livestock treatment due to the increased infections by antibiotic resistant strains. Antimicrobial peptides could be the alternate for treating such drug resistant strains. In the present study, the whey proteins extracted from buffalo milk was subjected to enzymatic hydrolysis using trypsin, chymotrypsin, pepsin...

background and objectives:   many surface proteins are implicated in nasopharyngeal colonization and pathogenesis of streptococcus pneumoniae . some of these factors are candidate antigens for protein based vaccines. new vaccine designs focus on the surface proteins (e. g., pspa and pspc ) and also cytolysin, and pneumolysin. in this study, 3 key virulence genes, lyta , pspc , and rrga , which ...

conclusions the results of the present study indicate that brazilian propolis demonstrated in vitro anti-microbial efficacy against s. mutans and c. albicans. results the antimicrobial efficacy of hexidine against s. mutans and e. coli was higher compared to that of c. albicans. the brazilian propolis (p) mr showed antimicrobial action against s. mutans and c. albicans. the antimicrobial effica...