Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw (LSL straw) for freezing few spermatozoa for intracytoplasmic sperm injection (ICSI). Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1:1, and then frozen using three different ...

Agriculture straw is considered a renewable resource that has the potential to contribute greatly to bioenergy supplies. Chemical pretreatment prior to anaerobic digestion can increase the anaerobic digestibility of agriculture straw. The present study investigated the effects of seven chemical pretreatments on the composition and methane yield of corn straw to assess their effectiveness of dig...

Integration of wheat straw for a biorefinery-based energy generation process by producing ethanol and biogas together with the production of high-protein fungal biomass (suitable for feed application) was the main focus of the present study. An edible ascomycete fungal strain Neurospora intermedia was used for the ethanol fermentation and subsequent biomass production from dilute phosphoric aci...

rams fed, during 3 successive periods, the following 3 isoenergetic, isonitrogenous diets. I: control straw + 150 g of grain corn and 135 g of soya cake per kg of straw; 11: NaOH-treated straw + 155 g of soya cake per kg of straw; III: urea-treated straw + 70 g of soya cake per kg of straw; with 20 g of 9/15 CM daily and water ad libitum. After a 20 d adaptation period during which the animals ...

Pretreatment technology is important to the direct methanation of straw. This study used fresh water, four bacterium agents (stem rot agent, "result" microbe decomposition agent, straw pretreatment composite bacterium agent, and complex microorganism agent), biogas slurry, and two chemical reagents (sodium hydroxide and urea) as pretreatment promoters. Different treatments were performed, and t...

Biosorbents are the natural origin adsorbents, which popularity in environmental engineering is steadily increasing due to their low price, ease of acquisition, and lack of the toxic properties. Presented research aimed to analyze the possibility of chemical modification of the straw, which is a characteristic waste in the Polish agriculture, to improve its biosorption properties with respect t...

An in vitro experiment was conducted to evaluate the effect of methylcellulose on the attachment of major cellulolytic bacteria on rice straw and its digestibility. Rice straw was incubated with ruminal mixture with or without 0.1% methylcellulose (MC). The attachment of F. succinogenes, R. flavefaciens and R. albus populations on rice straw was measured using real-time PCR with specific primer...

To improve the surface wetting properties of rice straw, effects of distilled water and four alkali pretreatment on the straw surface wettability were analyzed. The straw surface contact angle at different time points was measured by JC2000A contact angle measuring apparatus, optical microscope (OM) and scanning electron microscopy (SEM) combined with a linear regression technique for parameter...

A solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using Aspergillus niger ATCC 9142. The Plackett Burman design (PBD) of experiments was used to test the relative importance of the variables affecting production such as moisture content, age of spore, inoculum size, initial pH of substrate, methanol conce...