We have assessed the effects of intraplantar local anaesthetics (bupivacaine and lignocaine) on carrageenan-induced oedema, mechanical allodynia and spinal c-fos protein expression. Mechanical allodynia was evaluated using the vocalization threshold to paw pressure (VTPP) every 30 min until 60, 180 or 240 min after administration of carrageenan. Peripheral oedema, mechanical allodynia and spina...

After the biologically inactive precipitate was removed, the filtrate was adjusted to pH 5.0 which resulted in the formation of dark red precipitate of crude pradimicin. This solid was stirred in a mixture of BuOH-MeOH-1% NaCl (3 :1 :4, pH 2.0). The organic layer was separated and extracted with alkaline water (pH 8.0). The aqueous layer was adjusted to pH 2.0 and subjected to column chromatogr...

Fibronectin is an abundant, high molecular-weight, extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix (3,4). Fibronectin plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis. It is a highly affinity-adhesive protein that binds to numerous matrix macr...

Intratumoral injection of ultrasonically prepared emulsions of the synthetic glycolipid methly 6-O-(2-tetradecyl-3-hydroxyoctadecanoyl)-alpha-D-glucopyranoside (designated C39) induced complete regression of transplants of a syngeneic murine fibrosarcoma in most of the treated animals as did 6,6'-di-O(2-tetradecyl-3-hydroxyoctadecanoyl)-alpha,alpha,-trehalose (designated C76) in a previous stud...

This study focused on the effects of electro-oxidation combined with electro-Fenton process using boron-doped diamond (BDD) electrodes degradation biorefractory food colorant caramel. Aqueous solutions containing Caramel Class IV, III and Acid Orange 7 were comparatively treated by electrochemical advanced oxidation processes, including (EO), (EF) (EO + EF). The experiments performed in a stirr...

Hormone-sensitive lipase (HSL) catalyzes the hydrolysis of intramuscular triacylglycerols (IMTGs), but HSL regulation is poorly understood in skeletal muscle. The present study measured human skeletal muscle HSL activity at rest and during 120 min of cycling at 60% of peak O2 uptake. Several putative HSL regulators were also measured, including muscle long-chain fatty acyl-CoA (LCFA CoA) and fr...

The effects on orange juice batch pasteurization in an improved pilot-scale microwave (MW) oven was evaluated by monitoring pectin methyl-esterase (PME) activity, color, carotenoid compounds and vitamin C content. Trials were performed on stirred orange juice heated at different temperatures (60, 70, 75, and 85 degrees C) during batch process. MW pilot plant allowed real-time temperature contro...

A rapid method for the determination of Pu isotopes in a soil sample within 60 min of starting sample pretreatment was developed. The large reduction in the analysis time was attained by the rapid and perfect digestion of the sample using an alkaline fusion method with an induction heating machine. Pu concentrations were then determined by flow injection/ICP-MS using a solid extraction resin af...