in this paper, we introduce the new class of implicit l-stable generalized hybrid methods forthe numerical solution of first order initial value problems. we generalize the hybrid methodswith utilize ynv directly in the right hand side of classical hybrid methods. the numericalexperimentation showed that our method is considerably more efficient compared to wellknown methods used for the numer...

Two numerical methods are presented for the periodic initial-value problem of the long wave–short wave interaction equations describing the interaction between one long longitudinal wave and two short transverse waves propagating in a generalized elastic medium. The first one is the relaxation method, which is implicit with second-order accuracy in both space and time. The second one is the spl...

Isolated-probe PCR (IP-PCR) is a method that combines asymmetric PCR, unlabeled probes, and high-resolution DNA melting while maintaining a closed tube system. A double-stranded DNA (dsDNA) dye LCGreen I was used to detect the unlabeled probes. LCGreen I is also used to detect the 277-base pair PCR product peak as an internal amplification control. To accomplish this, IP-PCR separates the asymm...

We evaluated the use of an integrated cell culture-reverse transcription-PCR (ICC-RT-PCR) procedure coupled with nested PCR to detect human astroviruses, enteroviruses, and adenovirus types 40 and 41 in surface water samples that were collected and evaluated by using the Information Collection Rule (ICR) method. The results obtained with the ICC-RT-PCR-nested PCR method were compared to the res...

There are few copies of Borna disease virus (BDV) genome in peripheral blood mononuclear cells and no reliable standard reverse transcriptase-polymerase chain reaction (RT-PCR) method for the detection of BDV RNA, which is both highly sensitive and free of contamination. Single-step RT-PCR, in which both reverse transcription and amplification by Taq DNA polymerase work efficiently in a single ...