root rot caused by fusarium verticillioides is one of the most important rice diseases in ilam. in order to determine genetic diversity, 56 samples were collected from rice paddies of different regions in ilam province. molecular test was carried out with a set of five pairs of ssr primers after purification and identification of isolates. the ssr primers amplified a total 26 alleles. the avera...

We describe a technique, sequence-tagged microsatellite profiling (STMP), to rapidly generate large numbers of simple sequence repeat (SSR) markers from genomic or cDNA. This technique eliminates the need for library screening to identify SSR-containing clones and provides an approximately 25-fold increase in sequencing throughput compared to traditional methods. STMP generates short but charac...

Pummelo (Citrus grandis) is an important fruit crop worldwide because of its nutritional value. To accelerate the pummelo breeding program, it is essential to obtain extensive genetic information and develop relative molecular markers. Here, we obtained a 12-Gb transcriptome dataset of pummelo through a mixture of RNA from seven tissues using Illumina pair-end sequencing, assembled into 57,212 ...

Grevillea iaspicula is a highly restricted species occupying limestone outcrops near Wee Jasper, New South Wales (Australia). To allow future assessment of the ecological processes shaping the structure of G. iaspicula populations, seven polymorphic microsatellite (SSR) loci were developed from a partial genomic library. Amplification and polymorphism of six previously derived G. macleayana SSR...

UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed and characterized to analyze genetic diversity within Lagerstroemia cultivars and related species. • METHODS AND RESULTS Using simple sequence repeat (SSR)-enriched libraries, 11 species-specific polymorphic genomic SSRs were developed from L. indica 'Hong Die Fei Wu'. All primers were tested on 48 L. indica individuals ...

In order to support further genetic, diversity, and phylogeny studies of Capsicum species, the transferability of a Capsicum annuum L. simple sequence repeat (SSR) microsatellite set was analyzed for C. frutescens L. ("malagueta" and "tabasco" peppers) and C. chinense Jacq. (smell peppers, among other types). A total of 185 SSR primers were evaluated in 12 accessions from 115 C. frutescens L. a...