نتایج جستجو برای: sscp و pcr

تعداد نتایج: 925354  

Journal: :iranian journal of biotechnology 2009
azim mousavizadeh mohammadreza mohammad abadi azam torabi mohammad reza nassiry heydar ghiasi

the growth hormone gene could be an attractive candidate gene for milk production in goats. single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone (ggh) gene. for this purpose, genotyping of 90 talli goat breeds was performed. nine conformational patterns were observed in exon 4 of the ggh gene, with frequencies of 27.7% for the homozygous pattern (...

ژورنال: :زیست شناسی ایران 0

تحقیق حاضر به منظور بررسی پلی مورفیسم ژن bmp15 و تأثیر آن بر میزان دوقلوزایی و صفات رشد از تولد تا 15 ماهگی در گوسفند نژاد سنجابی انجام گرفت. برای انجام این پژوهش از 100 رأس گوسفند سنجابی (78 رأس میش و 22 رأس قوچ) ایستگاه اصلاح نژاد مهرگان کرمانشاه به طور تصادفی خون گیری انجام گرفت. بر اساس نتایج به دست آمده در جایگاه bmp15 80 درصد حیوانات ژنوتیپ mm و 20 درصد نیز ژنوتیپ nn را نشان دادند. در این...

ژورنال: :تحقیقات دام و طیور 0
فریبا سپهوند محمدتقی بیگی نصیری جمال فیاضی مجید خالداری

این تحقیق به منظور بررسی جهش در اینترون یک ژن میوستاتین در 110 رأس گوسفند نژاد لری انجام شد. قطعه 414 جفتبازی از اینترون یک ژن میوستاتین بهوسیله واکنش زنجیرهای پلیمراز- چندشکلی ساختار تکرشتهای (pcr-sscp) تکثیر و ارزیابی شد. برای جایگاه مذکور 5 الگوی sscp (ژنوتیپ) و 5 آلل مشاهده شد. فراوانی آللهای a، d، c، e و f به ترتیب برابر 69/0، 19/0، 07/0، 02/0 و 03/0 و فراوانی ژنوتیپهای aa، ad، ac، ae و af...

Journal: :jundishapur journal of microbiology 0
bahram nasr esfahani department of microbiology, isfahan university of medical sciences, isfahan, ir iran fatemeh sadat zarkesh department of microbiology, science and research branch, islamic azad university, fars, ir iran hadi rezaei yazdi department of microbiology, jahrom university of medical sciences, jahrom, ir iran tooba radaee department of microbiology, isfahan university of medical sciences, isfahan, ir iran; department of microbiology, isfahan university of medical sciences, isfahan, ir iran. tel: +98-3137922493, fax: +98-3136688597

conclusions we concluded from the results that the frequency of emb-resistant m. tuberculosis cases in iran is lower than that of many other regions. the pcr-sscp technique can separate resistant isolates from sensitive isolates. the sequencing results of this study showed mutation in codons 309 and 299 of the embb gene. in none of the resistant isolates, mutation was observed in codon 306. fur...

Journal: :Applied and environmental microbiology 2003
Markéta Derdáková Lorenza Beati Branislav Pet'ko Michal Stanko Durland Fish

In Europe the Borrelia burgdorferi sensu lato complex is represented by five distinct genospecies: Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. These taxonomic entities are known to differ in their specific associations with vertebrate hosts and to provoke distinct clinical manifestations in human patients. However, except...

Journal: :international journal of advanced biological and biomedical research 2013
maryam khatibi hedayatullah roshanfekr jamal fayazi khalil mirzade

lactoferrin is a glycoprotein with molecular weight 80 kda iron-binding bond, which is composed of 690 amino acids. in most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. this study was carried out to det...

Journal: :Zeitschrift fur Naturforschung. C, Journal of biosciences 2010
Eman M Gouda Gamal S Essawy

The insulin-like growth factor I (IGF-I) regulates growth, protein synthesis, and cell proliferation and differentiation in vertebrates. Polymorphisms of IGF-I gene transcripts of three breeds of chicken were assessed. The associations of these polymorphisms with the growth rate of the studied breeds were also evaluated. Total RNA was isolated from chicken livers, and the IGF-I gene was amplifi...

Journal: :Journal of biotechnology 2006
Sara Hortal Joan Pera Luis Galipienso Javier Parladé

Specific rDNA ITS amplifications, microsatellite-primed PCR and ITS-SSCP analysis were applied to identify and characterize pre-selected isolates of the edible ectomycorrhizal fungus Lactarius deliciosus in different stages of the life cycle. Sampling was performed from pure cultures, mycorrhizas and soil from experimental plots established with nursery-inoculated pine seedlings. A newly-design...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید باهنر کرمان - دانشکده کشاورزی 1392

این تحقیق با هدف بررسی چندشکلی ژن igfbp3 در گوسفند کرمانی با روش pcr-sscp انجام شد. در این پژوهش 90 راس گوسفند کرمانی به طور تصادفی مورد خون گیری قرار گرفتند. خون گیری با ونوجکت های edta دار انجام شد. استخراج dna از خون بوسیله کیت استاندارد صورت گرفت. سپس واکنش زنجیره ای پلی مراز (pcr) برای تکثیر قطعه 654 جفت بازی ژن igfbp3 انجام شد. برای تعیین چندشکلی نمونه ها، الکتروفورز محصولات pcr پس از تک ...

2016
Nesrin Turaçlar Hasibe Cingilli Vural Şahande Elagöz Emine Elif Altuntaş Fikriye Polat

The purpose of this study was to assess and explain the relationship between molecular changes occurring in squamous cell laryngeal carcinoma. Furthermore, we were revealed the role of FHIT gene response in the development and progression of squamous cell laryngeal carcinoma, and genes based exons 7,8 and 9 in tumour stage and histopathologic grade. In this study was used 50 blood samples for c...

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