Zafirlukast (ZAF) is a leukotriene receptor antagonist used in the treatment of chronic asthma. In this study, a simple and sensitive reversed-phase, high-performance liquid chromatographic method was developed for the determination of ZAF in pharmaceutical formulations and human plasma. Piribedil was used as an internal standard. Analysis was carried out on a Nucleosil C18 100 A (150 mm x 4.6 ...

introduction: water is an essential nutrient for life. it comprises 75% of total body weight in infants,60% in adult males and 50% in adult females. decrease in body water is commonly known as dehydration. acute or chronic dehydration is a common condition in some population groups, especialy the elderly and those who participate in physical activity in warm enviroments. potential consequences ...

Abstract Background An estimated >710 000 individuals live with bladder cancer in the United States, approximately 82 290 new cases expected this year (2023). Recurrence rates for are high: 65% of patients non-invasive or situ tumors and 73% more advanced disease experience recurrence within five years. Consequently, treated require prolonged surveillance to safeguard against tumor recur...

A spectrofluorimetric method to determine tacrine is proposed and applied to the determination of tacrine in human serum and pharmaceuticals. The fluorimetric method allows the determination of 1-70 ng ml-1 of tacrine in aqueous solutions containing acetic acid-sodium acetate buffer (pH 5.6) with lambda exc = 242 nm and lambda em = 362 nm.

Paper-based microfluidic devices are an attractive option for developing low-cost, point-of-care diagnostic tools. To incorporate more complex assays into paper, these must become sophisticated, through the sequential delivery of different liquids or reagents without user intervention. Many flow control strategies focus on slowing fluid down. However, this can lead to increased assay times and ...

The indole alkaloid, mitragynine, is the prominent substance in Mitragyna speciosa Korth. (kratom), use of which has led to addiction potential and adverse health effects. In this study, an electrochemical immunosensor was developed for first time sensitive rapid detection mitragynine. Multiwalled carbon nanotubes (MWCNTs)/ chitosan (CS) nanocomposite utilised as modifier sensor fabrication. mi...

Fumonisins are mycotoxins produced by Fusarium spp. and commonly contaminate maize and maize products worldwide. Fumonisins are rodent carcinogens and have been associated with human esophageal cancer. However, the lack of a valid exposure biomarker has hindered both the assessment of human exposure and the evaluation of disease risk. A sensitive liquid chromatography-mass spectrometry method t...

In a recent Brief Communication, Calafat et al. expressed concern that epidemiological studies inappropriately assess exposure to nonpersistent chemicals such as bisphenol A (BPA) and phthalates by measuring chemical concentrations in serum and tissues. They assert that urine is the most scientifically valid matrix and that accurate measurement of other matrices is difficult due to contaminatio...

The sensitivity and reliability of PCR for diagnostic and research purposes require efficient unbiased procedures of extraction and purification of nucleic acids. One of the major limitations of PCR-based tests is the inhibition of the amplification process by substances present in clinical samples. This study used specimens spiked with a known amount of plasmid pBKV (ATCC 33-1) to compare six ...