The aim of the study was to evaluate motility parameters of Lacaune (Lc) and east-Friesian (eF) ram frozen-thawed spermatozoa by cASA (computer-Automated Sperm Analysis) technique in relation to their in vivo fertilizing ability. Fresh ejaculates were diluted in a Triladyl extender containing 20 % egg yolk, 6 % glycerol and 0.1 % trehalose and frozen in a liquid nitrogen vapour. The evaluation ...

The responses of cane toad (Bufo marinus) gametes, used as a model for the development of assisted reproduction techniques for rare and endangered amphibians, to short-term storage at temperatures > 0 degrees C were studied. Whole excised testes were stored at 0 degrees or 4 degrees C for 15 days, and sperm motility was measured at excision and after storage for 2, 5, 7, 10, 12 and 15 days. Spe...

OBJECTIVE To investigate the causal role of oxidative-stress status on human sperm motility. DESIGN To demonstrate that sperm with higher oxidative damage have a lower antioxidant capacity. SETTING University hospital infertility center. PATIENT(S) Seventy-eight semen samples were obtained from 35 healthy donors who had normal semen characteristics and from 43 infertile or subfertile male...

Motility of spermatozoa was studied on 12 and 7 specimens of Acipenser persicus and A. stellatus, respectively. The density measured to be 2.22±0.65x109ml-1 in A. persicus and 2.21±0.55x109ml-1 in A. stellatus. Semen samples were diluted with two extenders containing tris 118mM, sucrose 23.4mM, pH=8, egg yolk (20%), dimethyl sulfoxide (15%) and penicillin potassium (500IU/ml) and biociphus exte...

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...

The aim of the present study was microbiological investigation of cervical samples of females suffering from unexplained infertility. 16 women were evaluated by standard bacterial culture method. Among total cases, all showed at least one type of bacteria. In total 27 isolates were obtained and most of these bacteria were isolated several times from different specimens. Staphylococcus was the m...

The use of deep-frozen boar semen for artificial insemination (AI) is constrained by the need for high sperm numbers per dose, yielding few doses per ejaculate. With the advancement of new, intra-uterine insemination strategies, there is an opportunity for freezing small volumes containing high sperm numbers, provided the spermatozoa properly sustain cryopreservation. The present study aimed to...

In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potent...

The aim of the present study was to assess the effect of addition of Royal jelly in presence of heparin on buffalo (Bubalus Bubalis) sperm motility, acrosome reaction and in vitro fertilization (IVF) of buffalo oocytes. Frozen buffalo spermatozoa from five bulls were thawed and motile fraction was obtained by swim up technique. The spermatozoa were washed, treated with100 μg/ml heparin, and the...

Spermatozoa mediated gene transfer has become a promising technology to generate transgenic animals with disease resistance. However, exogenous DNA invasion may cause changes in spermatozoon natural defense system which result in spermatozoon dysfunction.The objective of this study was to investigate the changes of mitochondrial function and motility in goat spermatozoa after pre-incubation and...