Background: ROS production is a well-established phenomenon during sperm cryopreservation. Therefore, supplementation of freezing media with antioxidant is an inevitable event. The aim of this study was to assess whether a cell permeable superoxide dismutase agent such as MnTE, can further improve the quality of frozen/ thawed semen sample using a commercially optimized sperm cryopreservation m...

to compare the reproductive performance of the wild and cultured stocks of beluga, huso huso males, some spermatological parameters were measured including: sperm motility, sperm density, spermatocrit and also fertilization rate, hatching rate and larval growth and survival rates. the semen samples were sampled from 11 wild spawners and 12 cultured brooders. a half of each semen sample was allo...

Routine semen analysis in an infertile patient revealed severe teratospermia associated with malformation of head and tail in 100% of the sperm cells. Flow cytometry and fluorescence in-situ hybridization (FISH) were shown to supplement routine semen analysis by providing information on the sperm chromatin. Using flow cytometry, propidium iodide-stained spermatozoa from the same sperm sample we...

Objectives—To confirm or refute the hypothesis that organic farmers have higher sperm concentrations than traditional farmers. Methods—Traditional and organic farmers were selected randomly from central registers, and 171 traditional farmers and 85 organic farmers delivered one semen sample before the start of the spraying season. The participation rate was 28.8% among traditional farmers and 4...

The hypothesis to be tested was that abnormal sperm chromatin structure is related to disturbed spermatogenesis in patients with testicular cancer. After orchiectomy but before further treatment ("pretreatment"), semen samples from 39 patients with testicular cancer were analyzed for sperm concentration by light microscopy and by the sperm chromatin structure assay (SCSA). In 28 patients assess...

The aim of this study was to cryopreserve and evaluate frozen-thawed semen parameters. Electroejaculator was used to collect semen from six bucks. Semen samples were divided into two parts. In one part, seminal plasma was removed and was not in the other one. Both semen sample parts were extended with egg-yolk Tris extender. Semen was thereafter cryopreserved in straws using liquid nitrogen. Da...

Approximately 8 percent of couples in the World have problem with offspring production. Male infertility, which is the reason of the half of reproduction failures, is connected with diminished sperm production and deterioration of its quality. One important factor which affects fertility is the appearance of DNA damage in germ cells leading to enhanced frequency of mutations. This study aimed t...

The aim. aim of the work was to study indicators spermatogenesis in lake frog, Pelophylax ridibundus (Pallas, 1771), zone influence emissions from Burshtyn Thermal Power Plant (TPP).
 Materials and methods. Urinal sperm testes samples were taken male frogs under conditions anthropogenic pollution (Burshtyn industrial area). Such parameters as concentration ejaculate, number live spermatozo...

STUDY QUESTION Are urinary phthalate concentrations associated with altered semen quality parameters among males recruited from the general population? SUMMARY ANSWER Urinary levels of metabolites of phthalate diesters are associated with lower total sperm counts, larger sperm head sizes, and higher percentages of morphologically abnormal sperm. WHAT IS KNOWN ALREADY High dose experiments i...

OBJECTIVE Sperm banking is an effective method to preserve fertility, but is not universally offered to males facing gonadotoxic treatment in the United States. We compared the disposition and semen parameters of cryopreserved sperm from individuals referred for sperm banking secondary to a cancer diagnosis to those of sperm from men banking for infertility reasons. STUDY DESIGN We performed ...