Variation in the effect of seminal plasma on sperm function and fertility has been hypothesised to be due to differences between males and their seminal plasma composition. The freezing resilience of individual rams (n=17) was investigated to characterise inter-male variation. This was determined by measuring the degree of change in motility induced by cryopreservation (Experiment 1). Experimen...

Aim: The study was undertaken to quantify and to analyze the changes in the motion characteristics of bull spermatozoa during various stages of cryopreservation cycle. Materials and Methods: Using computer assisted semen analysis (CASA) technique, 26 ejaculates, collected from two Jersey bulls were analyzed for motility, head behaviour and swimming pattern of spermatozoa on dilution, pre-freeze...

Caffeine was incorporated in egg yolk tris glycerol extender (EYTG) at the time of dilution of Murrah semen at a concentration of 1.0, 3.0 or 5.0 mM. The samples were subsequently frozen. The post thaw progressive motility and live sperm per cent improved signifi cantly (P<0.05) with addition of 1.0 (70.0±0.99 and 88.80±0.84) and 3.0 mΜ (65.83±0.88 and 86.16±0.96%) caffeine to the extender befo...

The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen wa...

There are various advantages, such as the preservation of genetic resources, an improvement of the reproduction performance in summer, long-distance transportation of semen and the possibility of progeny test, in the cryopreservation technique of boar semen. However, cryopreserved boar spermatozoa have not been routinely available to swine artificial insemination (AI) due to the lower conceptio...

Egg yolk (EY) protects cell membranes against cold shock, and it prevents or restores the loss of phospholipids from the membrane. EY has been widely used in semen extenders. It has been added to Tris-Glucose buffer and has been widely used for cooling and cryopreservation of canine semen. EY is not a defined entity, but a complex biological compound containing proteins, vitamins, phospholipids...

Cryopreservation causes extensive damage to spermatozoa, thereby impairing their fertilizing ability. The purpose of this study was to determine if the direct addition of pentoxifylline to the seminal plasma before cryopreservation improved sperm motility and acrosome reaction. Semen specimens from 15 healthy volunteers were divided into two aliquots. One aliquot was treated by adding 5 mM pent...

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

BACKGROUND Some types of cancer treatment entail a risk of reduced fertility and infertility. Fertility-preserving treatment can reduce the risk for some. The purpose of this article is to provide an overview of the risk of infertility after treatment of boys and young men with cancer and of fertility-preserving measures. MATERIAL AND METHODS The article is based on literature searches in the...