Surface-enhanced Raman spectroscopy (SERS) is a promising tool in the analytical science because it provides good selectivity and sensitivity without the labeling process required by fluorescence detection. This technique consists of locating the target analyte on nanometer range of roughed Au-nanoparticles. The presence of the metal nanoparticles provides a tremendous enhancement to the result...

The present study was undertaken to develop a suitable transdermal matrix patch of ketorolac tromethamine with different proportions of polyvinyl pyrrolidone (PVP) and ethyl cellulose (EC) using a D-optimal mixture design. The prepared transdermal patches were subjected to different physicochemical evaluation. The surface topography of the patches was examined by scanning electron microscopy (S...

Low-coherence enhanced backscattering (LEBS) spectroscopy is a light scattering technique which uses partial spatial coherence broadband illumination to interrogate the optical properties at sub-diffusion length scales. In this work, we present a post-processing technique which isolates the hemoglobin concentration at different depths within a sample using a single spectroscopic LEBS measuremen...

We demonstrate a new nanoscale spectroscopic technique that combines subwavelength near-field imaging with broadband interference spectroscopy. We apply this technique to study phase spectra of surface plasmons in individual gold nanoparticles and nanoparticle dimers. Collective plasmon oscillations in selected nanostructures are excited by a femtosecond white-light continuum transmitted throug...

The analysis of kinetic data monitored using spectroscopic techniques and its resolution into its unknown components is described. Independent Component Analysis (ICA) can be considered a calibration free technique with the outcome of the analyses being the spectral profiles of the unknown species. This enables the realisation of qualitative information concerning the identification of the numb...

An infrared (IR) spectroscopic technique was used to determine the possible functional groups and chemical compounds present in commercially available ink-jet printing reactive dyes of four primary colours, i.e. Cyan, Magenta, Yellow, and Black. Although other instrumental analytical methods can help to determine the actual chemical composition of reactive dyes, the IR technique alone can still...

Chemical imaging is a technique for the simultaneous measurement of spectra (chemical information) and images or pictures (spatial information)[1][2] The technique is most often applied to either solid or gel samples, and has applications in chemistry, biology[3][4][5] [6][7][8], medicine[9][10], pharmacy[11] (see also for example: Chemical Imaging Without Dyeing), food science, biotechnology[1...

A two-dimensional spectroscopic technique is presented. Application of the technique to determine all the relaxation rates for a multi-level quadrupole system is discussed along with experimental requirements. Theoretical and experimental data are analyzed for theN, three level, spin 1 system. The analysis shows that all three relaxation rates can be obtained from a single two-dimensional spect...

We have developed a multifocus confocal Raman microspectroscopy system that allows simultaneous analyses of ≈ 80 individual biological or airborne microparticles based on a precise image-guided technique. Multiple individual particles adhered in random positions on a coverslip were illuminated by a multifocus excitation pattern formed by rapidly steering a single laser beam with a pair of galvo...

We develop a new method for estimating and removing the spectrum of the sky from deep spectroscopic observations. Our method does not rely on simultaneous measurement of the sky spectrum with the object spectrum. The technique is based on the iterative subtraction of continuum estimates and eigenvector sky models derived from singular value decompositions of sky spectra and sky spectra residual...