a simple and sensitive method is described for the pre-concentration by cloud point extraction followed by atomic absorption spectrometric determination of copper ion in real samples. at a solution ph of 5.5, 5, 5-diphenylimidazolidine-2, 4-dione (phenytoin) (dfid) and tritonx-114 were used as hydrophobic ligand and non-ionic surfactant. the adopted concentrations for dfid, triton x-114 and hno...

The general theme of the meeting was the application of mass spectrometry to pharmaceutical and biotechnological research. The majority of the oral presentations and posters were concerned with the development and application of all mass spectrometric techniques related to proteomics.

Airborne particulate matter (PM10 ) was collected from the atmosphere of the city of Isfahan. The concentration of heavy metals and anions associated with airborne particulate matter were determined using atomic absorption spectrometric and ion chromatographic techniques. A comparison was made between the variation in the concentration of PM10 and that for heavy metals and...

A new approach to mass-spectrometric determination of the combined isotopic composition methane has been put forward. The is based on preliminary on-line conversion methanol in corona discharge and selective chemical ionization. provides means lower requirements for equipment, consumables, operation conditions offers a challenge develop field method.

introduction iron ore is one of the most important natural raw materials that is widely used for manufacturing iron and steel. this type of ore contains various amounts of radionuclides; thus, exposing workers handling their extraction, transportation, and processing to radiation. materials and methods in this study, 12 ore samples (each mass weighing about 2 kg) were collected from the iron or...

Most methods for the analysis of oligosaccharides from biological sources require a glycan derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore, facilitating detection after chromatographic or electrophoretic separation. Derivatization can also be applied to link charged or hydrophobic groups at the reducing end to enhance glycan separation and mass-spectrom...

Quantitative proteomics involves the identification and quantitation of protein components in various biological systems. Stable isotope labelling technology, by both metabolic and chemical methods, has been the most commonly used approach for global proteome-wide profiling. Recently, its capability has been extended from labelled pairs to multiple labels, allowing for the simultaneous quantifi...

A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The analyte and internal standard, nevirapine, were extracted from plasma matrix by liquid-liquid extraction with ethyl acetate. Chromatographic separation was achieved on a C-18 analytical column with an isocratic mobile phase of 15:85 (V/V)...

We have developed a simple method for the release and isolation of glycoprotein N-glycans from whole-cell lysates using less than a million cells, for subsequent implementation with mass spectrometric analysis. Cellular protein extracts prepared using SDS solubilization were sequentially treated in a membrane filter device to ultimately release glycans enzymatically using PNGase F in the volati...