During the past few years several investigators have reported the isolation of lactobacilli or lactobacilli-like organisms from the rumen liquor of cattle and sheep. Gall and Huhtanen (1951) isolated several gram positive, nonsporeforming rods from rumen fluid which produced lactic acid from glucose, but they were not further identified. Some lactobacilli found by Rodwell (1953) in a sheep's ru...

The objective of this study was to investigate the effects of diets containing different levels of milk thistle (0, 100 and 200 g/kg dry matter) and grains (barley and corn) with different rumen degradation rates on rumen bacteria and whole rumen microorganisms (WRM) of Khuzestan buffalo. The gas production (GP) technique, two steps digestion and specific bacteria culture medium methods were us...

BACKGROUND The rumen harbors a complex microbial ecosystem for efficient hydrolysis of plant polysaccharides which are the main constituent of the diet. Xylanase is crucial for hemicellulose hydrolysis and plays an important role in the plant cell wall degradation. Xylanases of ruminal strains were widely studied, but few studies have focused on their diversity in rumen microenvironment. METH...

Bacteria make up about half of the living organisms inside of the rumen. However, they do more than half of the work in the rumen. The bacteria work together. Some breakdown certain carbohydrates and proteins which are then used by others. Some require certain growth factors, such as B-vitamins, which are made by others. Some bacteria help to clean up the rumen of others’ end products, such as ...

Bacteria make up about half of the living organisms inside of the rumen. However, they do more than half of the work in the rumen. The bacteria work together. Some breakdown certain carbohydrates and proteins which are then used by others. Some require certain growth factors, such as B-vitamins, which are made by others. Some bacteria help to clean up the rumen of others’ end products, such as ...

This study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. The community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and Illumina high-throughput sequencing, respectively. Chemical oxygen demand eff...

Dichloromethane (DCM) is utilized by the strictly anaerobic, acetogenic mixed culture DM as a sole source of carbon and energy for growth. Growth with DCM was linear, and cell suspensions of the culture degraded DCM with a specific activity of 0.47 mkat/kg of protein. A mass balance of 2 mol of chloride and 0.42 mol of acetate per mol of DCM was observed. The dehalogenation reaction showed simi...

BACKGROUND Efficient conversion of lignocellulosic biomass to fermentable sugars requires the synergistic action of multiple enzymes; consequently enzyme mixtures must be properly formulated for effective hydrolysis. The nature of an optimal enzyme blends depends on the type of pretreatment employed as well the characteristics of the substrate. In this study, statistical experimental design was...

BACKGROUND AND OBJECTIVES Essential oils may improve the utilization of nutrients by ruminal microorganisms. The aim of this study was to assess the effect of different doses of ajowan essential oils (AEO) on growth and fibrolytic activity of anaerobic fungi, and generic distribution of ciliated protozoa (in vitro). MATERIAL AND METHODS Different doses of AEO (0, 150, 300, 450 and 600 ppm) we...

Biogas productions of cattle manure using rumen fluid inoculums were determined using batch anaerobic digesters at mesophilic temperatures (room and 38.5 C). The aim of this paper was to analyze the influence of rumen fluid contents on biogas yield from cattle manure using fluid rumen inoculums. A series of laboratory experiments using 400 ml biodigester were performed in batch operation mode. ...