After several decades of research on the subject, we now know when the first livestock reached southern Africa but the question of how they got there remains a contentious topic. Debate centres on whether they were brought with a large migration of Khoe-speakers who originated from East Africa; or whether the livestock were traded down-the-line among hunter-gatherer communities; or indeed wheth...

CHAMP satellite and relatively densely spaced ground-based data measured over southern Africa between 2005 and 2010 are combined in a new regional, harmonic spline based, core field model. This new SACFM-2 model is compared to the regional SARM model, which is based only on satellite data, and the global CHAOS-6 model. The results agree well in the vertical (Z) component, with somewhat larger d...

Recent estimates suggest that more than 10% of Southern Africa is HIV+. This article is based on a research study of sixty four pastoral care projects focussing on the HIV/AIDS pandemic in Southern Africa. The projects, which fall under the auspices of the Southern African Catholic Bishops' Conference AIDS office, focus on both education for HIV prevention and the pastoral care of people living...

Migration has deep historical roots in South and southern Africa and to this day continues to be highly prevalent and a major factor shaping South African society and health. In this paper we examine the role of migration in the spread of two diseases nearly 100 years apart: tuberculosis following the discovery of gold in 1886 and HIV in the early 1990s. Both cases demonstrate the critical role...

The polymerase chain reaction (PCR) was investigated for detecting human parvovirus B19 (B19) DNA in sera. Three pairs of oligonucleotides were evaluated as primers. The best oligonucleotide pair spanned 699 nucleotides, including the region common to VP1 and VP2. After PCR amplification of B19 DNA in serum, a 699-nucleotide DNA fragment was detected on agarose gels. This DNA fragment was B19 D...

introduction: l. pneumophila is the most important cause of legionaires, disease, which is currently reported either as a nosocomial or community acquired infection. rapid and reliable diagnosis of legionella has hampered the epidemiological studies and disease control activities. in spite of good sensitivity and specificity, the culture method encountered serious shortcomings. polymerase chain...

PURPOSE To establish the Southern blotting technique using hybridization with a nonradioactive probe to detect large rearrangements of CYP21A2 in a Brazilian cohort with congenital adrenal hyperplasia due to 21-hydroxylase deficiency (CAH-21OH). METHOD We studied 42 patients, 2 of them related, comprising 80 non-related alleles. DNA samples were obtained from peripheral blood, digested by res...