Enniatins are cyclic depsipeptides produced by fungi of the genus Fusarium that are of interest due to their pronounced biological activities; especially enniatins A, A1, B, and B1 possess anticarcinogenic and anti-HIV properties. In the present study, F. tricinctum was grown on seven solid media and in one liquid growth medium with or without addition of peptone or of amino acid precursors in ...

We have investigated the first events that occur when exponentially grown cells are transferred from a liquid medium (Luria-Bertani [LB]) to a solid medium (LB agar [LBA]). We observed an initial lag phase of 180 min for the wild type MG1655 without any apparent growth. This lack of growth was independent of the bacterial physiological state (either the stationary or the exponential phase), the...

For a two-phase swelling porous medium, two pressures have been previously defined thermodynamically in terms of intensive variables for each phase: one relating the change in energy with respect to specific volume, and the other relating the change in energy with respect to volume fraction. Within the framework of Hybrid Mixture Theory and hence the Coleman and Noll technique of exploiting the...

Tunicamycin (Tm) sensitivity assays are a useful method for studies of endoplasmic reticulum stress and the unfolded protein response in eukaryotic cells. While Tm sensitivity and Tm recovery assays have been previously described, these existing methods are time-consuming, labor intensive, and subjected to mechanical wounding. This study shows an improved method of testing Tm sensitivity in Ara...

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + ric...

A circular gravity-phase separator using coalescing medium with cross flow was developed to remove oil and suspended solids from wastewaters. Coalescence medium in the form of inclined plates promotes rising of oil droplets through coalescence and settling of solid particles through coagulation. It exhibits 22.67% higher removal of total suspended solids (TSS) compared to separators without coa...

Elite genotypes from high yielding variety of Artemisia annua L. (Jeevanraksha, CIMAP Lucknow) were regenerated form nodal explants and maintained in MS culture medium with BAP and NAA. The leaves of tissue culture grown plants were inoculated with Agrobacterium rhizogenes strain LBA9402 containing the selective marker gene NPT II. Hairy roots emerged from the leaves, 8-10 days after inoculatio...

A defined solid and liquid minimal medium, HTM, which contained methionine and cysteine as the sole amino acids, was developed for Listeria monocytogenes. Complex broth-grown L. monocytogenes had to adapt to HTM by inducing amino acid biosyntheis. HTM is the simplest minimal medium available for growth of L. monocytogenes.

An improved motility medium which permits additional cultural characterization is described. Advantages include maximal motility due to a change in the physical state of the medium from solid to liquid at incubation temperatures, a definitive stab line, preservation of the stab line with nonmotile organisms, and visual delineation of culture motility. In addition, nitrate reduction, nitrogen ga...

Aseptic cultures of Maranta leuconeura were established from shoot tip explants. They were initiated on Murashige and Skoog (MS)-basal medium supplemented with benzyladenine (BA, 5 mg/l) and agar (6 mg/l) (starting medium). Explants were transferred at 6 week intervals until the onset of proliferation (ca. 6 months). Thereafter, four subcultures were made in liquid multiplication medium contain...