Urease is a nickel-containing urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism in plants. The process of urease activation by incorporation of nickel into the active site is a prime example of chaperone-mediated metal transfer to an enzyme. Four urease accessory proteins are required for activation in Klebsiella aerogenes. In plants urease accessory pro...

Ubiquitous soybean urease, as opposed to the seed-specific urease, designates the seemingly identical ureolytic activities of suspension cultures and leaves. It also appears to be the basal urease in developing seeds of a variety, Itachi, which lacks the seed-specific urease (Polacco, Winkler 1984 Plant Physiol 74: 800-804). On native polyacrylamide gels the ureolytic activities in crude extrac...

The important human pathogen Helicobacter pylori requires the abundant expression and activity of its urease enzyme for colonization of the gastric mucosa. The transcription, expression, and activity of H. pylori urease were previously demonstrated to be induced by nickel supplementation of growth media. Here it is demonstrated that the HP1338 protein, an ortholog of the Escherichia coli nickel...

The stability (longevity of activity) three crude urease extracts was evaluated in a laboratory study as part an effort to reduce the cost for applications that do not require high purity enzyme. A low-cost, stable source will greatly facilitate engineering such biocementation soil. Inexpensive have been shown be effective at hydrolyzing urea carbonate precipitation. However, some studies sugge...

UNLABELLED Urease in Cryptococcus neoformans plays an important role in fungal dissemination to the brain and causing meningoencephalitis. Although urea is not required for synthesis of apourease encoded by URE1, the available nitrogen source affected the expression of URE1 as well as the level of the enzyme activity. Activation of the apoenzyme requires three accessory proteins, Ure4, Ure6, an...

BACKGROUND Helicobacter pylori produces large amounts of urease presumably to be prepared for the rare event of a sudden acid exposure. The hypothesis that H pylori is acid sensitive and protein production is inhibited by low pH was examined. METHODS H pylori or its soluble enzymes were incubated buffered or unbuffered at a pH ranging from 2-7 in the presence of 5 mM urea for 30 minutes. Afte...

OBJECTIVE Urease enzymes produced by oral bacteria generate ammonia, which can have a significant impact on the oral ecology and, consequently, on oral health. To evaluate the relationship of urease with dental plaque microbial profiles in children as it relates to dental caries, and to identify the main contributors to this activity. METHODS 82 supragingival plaque samples were collected fro...

A large amount of plant pruning waste occurs after annual care in banana production areas. This material contains significant amounts organic substances and nutrients. In this study, compost (BWC) was applied to the soil both alone mixture with leonardite (LT) vinasse (VC). Treatments include: control (CL), (BWC-2: 2 t da-1; BWC-4: 4 BWC-8: 8 da-1), (LT: recommended application rate), (BWC-2+LT...

Urease from jack bean meal and hydrated seeds has been obtained in 25 to 33% yield with specific activity in the range of 1000 to 1070 units/mg protein. A purification of 100 to 130-fold was achieved from meal and fully soaked seeds. Use of beta-mercaptoethanol and EDTA was found essential to obtain this high yield and purity. Amino acid analysis showed all 18 amino acids commonly found in prot...