Two simple and rapid titrimetric methods are described for the determination of bupropion hydrochloride (BUPH) in pharmaceuticals. The proposed methods are based on the solvent extraction-titration of BUPH with two ion-association reagents, i.e., sodium lauryl sulphate (SLS) and tetraphenylborate (TPB). In method A, SLS was used as titrant and the titration was carried out in the presence of di...

The development of novel in vitro methods to assess risks of allergic sensitization are essential in reducing animal testing whilst maintaining consumer safety. The main research objectives of this study were to identify novel biomarkers to assess the sensitization predictability of chemicals. Phenotypic and cytokine responses of moDCs and MUTZ-3 cells were investigated following application of...

The present study describes the development and validation of a dissolution method for carvedilol compression-coated tablets. Dissolution test was performed using a TDT-06T dissolution apparatus. Based on the physiological conditions of the body, 0.1N hydrochloric acid was used as dissolution medium and release was monitored for 2 hours to verify the immediate release pattern of the drug in aci...

Patients with atopic skin show a defective barrier function both in rough and in clinically normal skin, with an increasing risk of developing contact dermatitis. Moisturizing creams are often used in the treatment of dry skin. The purpose of this study was to investigate the influence of treatment with a urea-containing moisturizer on the barrier properties of atopic skin. Fifteen patients wit...

Corneal tissue engineering has improved dramatically over recent years. It is now possible to apply these technological advancements to the development of superior in vitro ocular surface models to reduce animal testing. We aim to show the effect different substrates can have on the viability of expanded corneal epithelial cells and that those which more accurately mimic the stromal surface pro...

1. Aspartate-carbamoyltransferase activity was concentrated from rat-liver preparations. Only l-aspartate, beta-benzyl-l-aspartate and beta-erythro-hydroxy-dl-aspartate were carbamoylated enzymically. The K(m) for l-aspartate and carbamoyl phosphate have been determined by three methods: colorimetric procedure, radioactive assay with [(14)C]aspartate and an assay with [(14)C]carbamoyl phosphate...

A total of 335 quarter milk samples from crossbred dairy cows were subjected to California mastitis (CMT), sodium lauryl sulphate (SLST), and somatic cell count (SCC) tests. CMT positive milk samples were 227, while 108 samples were CMT negative. Milk samples positive by SLST and SCC were 261 and 180 respectively. The CMT positive samples were subjected to cultural isolation (gold standard test...

The molecular weight and hydrodynamic properties of a polypeptide isolated from the lamellar system of Antirrhinum chloroplasts were determined in sodium dodecyl sulphate solution by measurement of sedimentation velocity, diffusion and effective partial specific volume. The polypeptide fraction exhibits a molecular weight of 25000 which agrees with the apparent molecular weight found by polyacr...

PURPOSE The aim of this study was to formulate stable film-coated montelukast sodium (MS) tablets using Opadry(®) yellow 20A82938 (Montikast(®) tablets) and to evaluate their in vitro and in vivo release profile. METHODS MS core tablets were manufactured using a direct compression method. Opadry yellow 20A82938 aqueous coating dispersion was used as the film-coating material. Dissolution of t...