The importance of Web site usability is apparent. Previous studies have found that a usable Web site creates a positive attitude toward online stores, increases stickiness and revisit rates, and eventually stimulates online purchases. A usable Web site also provides benefits to e-business by reducing website development, support, and maintenance costs. The ISO 9241-11 standard for usability has...

This paper presents techniques for identifying domain specific web sites that have been implemented as part of the EC-funded R&D project, CROSSMARC. The project aims to develop technology for extracting interesting information from domain-specific web pages. It is therefore important for CROSSMARC to identify web sites in which interesting domain specific pages reside (focused web crawling). Th...

Reports Purfication of Antibody to Galactosyl-Protein Conjugates: E. W. Bassett, S. M. Beiser, S. W. Tanenbaum ...... .................................. 1475 Age Changes in Adenine Nucleotides in Flight Muscle of Male House Fly: M. Rockstein and D. E. Gutfreund ....... .............................. 1476 Ether-Induced Retrograde Amnesia for One-Trial Conditioning in Mice: J. P. Abt, W. B. Essma...

OBJECTIVES To assess patients' usage of the Internet as a source of personal healthcare information and patients' perceptions of usefulness and content preferences of more locally focused online health-related material. MATERIALS AND METHODS A paper-based survey was undertaken by a convenience sample of cardiac outpatients. Age, gender, Internet access, Internet usage, perception of usefulnes...

We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flanking primers and one mutagenic primer with different melting temperatures (T(m)) are used togethe...

BACKGROUND Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate purification of DNA between two rounds of PCR. RESULTS The strategy relies on the use of a limiting concentration of one of the flanking primers (reve...

BACKGROUND Health care professionals, trainees, and patients use the Internet extensively. Editable Web sites may contain inaccurate, incomplete, and/or outdated information that may mislead the public's perception of the topic. OBJECTIVE To evaluate the editable, online descriptions of clinical pharmacy and pharmacist and attempt to improve their accuracy. METHODS The authors identified ke...

An important step in the design process for a web site is to determine which information is to be included and how the information should be organized on the web site’s pages. In this paper we describe ’SiteGuide’, a tool that automatically produces an information architecture for a web site that a user wants to create. SiteGuide takes as input URLs of web sites from the same domain as this tar...

Here we report the use of exonuclease to expose complementary DNA between an insert and vector such that annealing becomes independent of restriction site compatibility. We demonstrate that unusual and, in some cases, previously impossible cloning strategies can be readily and efficiently achieved as long as the flanking sequences of the linear vectors are highly related. Furthermore, we show t...

The AASB intends to formally approve all UIG Abstracts that are equivalent to IASB Interpretations on the same day in 2004. The UIG intends to finalise the contents of Proposed Pending Abstracts on a progressive basis and to make them available on the AASB web site, to assist entities to identify changes that may be needed in their financial reporting systems. When agreed by the AASB, Pending A...