نتایج جستجو برای: sirna targeting cassette

تعداد نتایج: 175780  

2015
Guo-Wei Huang Lian-Di Liao En-Min Li Li-Yan Xu

Recent studies show that targeting gene promoter or 3' terminal regions of mRNA with siRNA induces target gene transcription. However, the ability of exon-targeting siRNA to affect transcription has yet to be demonstrated. We designed and synthesized siRNA against various exons in the gelsolin gene (GSN) to knockdown GSN transcript in KYSE150 and KYSE450 cells. Surprisingly, we found that siGSN...

Journal: Nanomedicine Journal 2015
Ali Badiee, Fatemeh mosaffa Hermann Lage Javad Behravan, Khalil Abnous, Mahmoud R. Jaafari Mahnaz Nourbakhsh

Objective(s): Lipid-based nanoparticles (NLP) are PEGylated carriers composed of lipids and encapsulated nucleic acids with a diameter less than 100 nm. The presence of PEG in the NLP formulation improves the particle pharmacokinetic behavior. The purpose of this study was to prepare and characterize NLPs containing MDR1 siRNA and evaluate their cytotoxicity and cellular uptake. MDR1 siRNA coul...

Journal: :PLoS Genetics 2008
Kun Yu Kumaresan Ganesan Lay Keng Tan Mirtha Laban Jeanie Wu Xiao Dong Zhao Hongmin Li Carol Ho Wing Leung Yansong Zhu Chia Lin Wei Shing Chuan Hooi Lance Miller Patrick Tan

Successful tumor development and progression involves the complex interplay of both pro- and anti-oncogenic signaling pathways. Genetic components balancing these opposing activities are likely to require tight regulation, because even subtle alterations in their expression may disrupt this balance with major consequences for various cancer-associated phenotypes. Here, we describe a cassette of...

2010
Hehua Ye Yiyong Qian Mingkai Lin Yongheng Duan Xuerong Sun Yehong Zhuo Jian Ge

PURPOSE To investigate the efficacy and safety of cationic nano-copolymers CS-g-(PEI-b-mPEG) mediated IκB kinase beta (IKKβ) targeting siRNA in modulating wound healing in a monkey model of glaucoma filtration surgery. METHODS The IKKβ targeting siRNAs were chemically synthesized and screened in cultured monkey Tenon's fibroblasts in vitro. Fourteen monkeys underwent trabeculectomy and were r...

Journal: :Oncology reports 2012
Xin Zhao De-Chun Li Hua Zhao Zhi Li Jian-Xin Wang Dong-Ming Zhu Jin Zhou Jian-Nong Cen

The non-receptor protein tyrosine kinase c-Src regulates diverse biological processes by associating with multiple signaling and structural molecules. Overexpression of c-Src occurs in pancreatic cancer and can be predictive of poor prognosis. The aim of this study was to investigate the inhibitory effects of plasmid-based siRNAs targeting the hum...

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