DT40 cells derived from chicken B lymphocytes exhibit exceptionally high homologous recombination rates. Therefore, they can be used as a convenient tool and model for gene targeting experiments. However, lack of efficient cloning strategies, protein purification protocols and a well annotated protein database limits the utility of these cells for proteomic studies. Here we describe a fast and ...

Using the command line interface can be intimidating for novice users. Primarily designed for seasoned users, others who could potentially benefit from using it get stuck in its awkward and unfamiliar interface. The addition of “training wheels” has the potential to help such learners begin to “ride” the command line without “falling off” and getting discouraged. This paper proposes a "training...

The notion of singular limiting induced from continuum solutions (slic-solutions) is applied to the problem of cavitation in nonlinear elasticity, in order to re-assess an example of non-uniqueness of entropic weak solutions (with polyconvex energy) due to a forming cavity.

The design of an interface unit is described, in which RS232 serial data is converted to latched parallel data on 22 independent lines. The data direction of each line is programmable through the serial port. Two picocontrollers are employed in a parallel processing mode to give the required number of I/O pins, and data on the shared serial line is coded to separate data streams to the individu...

The article describes a way of evaluating the power line channel frequency response and input impedance by means of the linear time-invariant (LTI) power line generator. Two possible methods are introduced for the calculation of primary parameters: the first method depends on the physical realization and physical dimension of the cable, and the second method is derived from the data provided by...

This paper describes a simple-yet-effective for a DC-DC buck converter with voltag (VMC). We propose to use a slope adjustable generator (TWG) which provides a line feed and an additional duty cycle modulatio comparing with the conventional VMC, the p significantly improves the line and load tran This triangular wave slope regulation sc compared to digital feed-forward control sch non-linear ca...

Line Integral Convolution (LIC) is a common approach for the visualization of 2 0 vector fields. It is well suited for visualizing the direction of a flow field, but it gives no information about the orientation of the underlying vectors. We introduce Oriented Line Integral Convolution (OLIC), where direction as well as orientation are encoded within the resulting image. This is achieved b y us...

Line Integral Convolution (LIC) is a common approach for the visualization of vector elds. It is well suited for visualizing the direction of a ow eld, but it gives no information about the orientation of the underlying vectors. We introduce Oriented Line Integral Convolution (OLIC), where direction as well as orientation are encoded within the resulting image. This is achieved by using a low f...

A new finite element level set method is developed to simulate the interface motion. The normal velocity of the moving interface can depend on both the local geometry, such as the curvature, and the external force such as that due to the flux from both sides of the interface of a material whose concentration is governed by a diffusion equation. The key idea of the method is to use an interface-...

A physically plausible lower bound on the spatially varying geostrophic mesoscale eddy energy dissipation time-scale within ocean, related to geographical transfer rate out of mesoscales, is provided by means a simple and computational inexpensive inverse calculation. Data diagnosed from high resolution global configuration ocean simulation supplied parameterized model energy, which results as ...