The aim of the study is to develop and validate a simple, accurate, precise and rapid isocratic reverse-phase high-performance liquid chromatographic method for simultaneous determination of Elbasvir and Grazoprevir in bulk and tablet formulations. Chromatographic separation was supported on Inertsil ODS, 5μm C18(150x4.6 ID) column with a blend of methanol: water (80:20) as mobile phase at a fl...

Pharmaceutical compounds represent a rapidly emerging class of environmental contaminants. Such compounds were recently classified by the U.S. Geological Survey, including several antibiotics. An LC-MS/MS screening method for the top five antibiotics in drinking water was developed and validated using a Shimadzu LC-MS-IT-TOF. The separation was performed using a Waters Acquity UPLC BEH C18 colu...

A technology to characterize early enamel lesions is needed in dentistry. Optical coherence tomography (OCT) is a noninvasive method that provides high-resolution cross-sectional images. The aim of this study is to compare OCT with microfocus x-ray computed tomography ([Formula: see text]) for assessment of natural enamel lesions in vitro. Ten human teeth with visible white spot-like changes on...

With the recent increase and awareness of natural rubber latex protein allergies, it is very important for all health care workers to understand and recognize the need to decrease and/or eliminate latex exposure in the rubber gloves and other rubber products for those individuals who are allergic to natural rubber latex. This study is based on a series of Lowry method of protein analysis. The f...

OBJECTIVES 1-hydroxypyrene is an important biomarker of exposure to polycyclic aromatic hydrocarbons (PAHs), which appears in the urine of exposed human subjects. In developing countries, where advanced instruments are not available, the importance of this biomarker demands convenient and sensitive methods for determination purposes. This study aimed at developing a methodology to quantify 1-hy...

Objective: To investigate the correlation of the immunoassay and chromatography method for quantitative measurement of two antiepileptic drugs (AED), carbamazepine (CBZ) and phenytoin (PHT) and determination of relation between the CBZ and it’s metabolite carbamazepine 10,11epoxide (CBZ-E). Additionally we investigated whether there is a di4 erence in the determination of serum concentration of...

India is one of the richest known in terms biodiversity, especially northern Jammu and Kashmir beautiful geographical regions world hub medicinal plants. Two plants namely Glycyrrhiza glabra L. Malwa sylvestris were selected for study. The aim this research was to investigate presence preliminary phytochemicals determine total flavonoid phenolic content. Isolation phytoconstituents analyzed thr...

Objective: The main objective of this study is to develop and validate a simple, new, fast, sensitive, precise accurate RP-HPLC analytical methods have been established for the estimation enrofloxacin in bulk pharmaceutical dosage forms.Methods: present method was developed validated on Waters HPLC system using Phenomenex make Shimadzu C18 column (250 mm × 4.6 i.d., 5μm particle size) used sepa...

Background: MSG has become one of the most widely used food flavorings in world, FDA (Food and Drug Administration) reported an average per capita intake United States 550 mg / day 1973. The use as a additive been regulated by its use. WHO. This rule stipulates that daily consumption person should not exceed safe threshold 120 kg day. is proven to be risk factor for obesity. increase body weigh...

Introduction: The current study aimed to develop and assess UV-spectrophotometric (zero order, first second area under the curve) RP-HPLC methods for estimating theophylline in its pharmaceutical dosage form.
 Methods: A less toxic solvent composition of acetonitrile 0.1% orthophosphoric acid (25:75 v/v) is used as a mobile phase diluent developing both UV-spectroscopic techniques. Shimadz...