Escherichia coli is a common cause of postweaning diarrhea in swine, but usually does not affect grower-finisher pigs. Eleven-week-old grower pigs presented with an acute and severe watery diarrhea, initially considered to be transmissible gastroenteritis. Diagnostic submissions showed the causative agent to be an F18positive E coli producing shiga-like toxin. No pigs developed neurological sig...

We have isolated Shiga toxin (Stx)-producing Escherichia coli (STEC) strains from the feces of feral pigeons which contained a new Stx2 variant gene designated stx(2f). This gene is most similar to sltIIva of patient E. coli O128:B12 isolate H.I.8. Stx2f reacted only weakly with commercial immunoassays. The prevalence of STEC organisms carrying the stx(2f) gene in pigeon droppings was 12.5%. Th...

An automated fluorescence-based PCR system (a model AG-9600 AmpliSensor analyzer) was investigated to determine whether it could detect Shiga toxin-producing Escherichia coli (STEC). The AmpliSensor PCR assay involves amplification-mediated disruption of a fluorogenic DNA signal duplex (AmpliSensor) that is homologous to conserved target sequences in a 323-bp amplified fragment of Shiga toxin g...

In July 2014, an outbreak of Shiga toxin-producing Escherichia coli (STEC) O55:H7 in England involved 31 patients, 13 (42%) of whom had hemolytic uremic syndrome. Isolates were sequenced, and the sequences were compared with publicly available sequences of E. coli O55:H7 and O157:H7. A core-genome phylogeny of the evolutionary history of the STEC O55:H7 outbreak strain revealed that the most pa...

BACKGROUND Ruminants, in particular bovines, are the primary reservoir of Shiga toxin-producing E. coli (STEC), but whole genome analyses of the current German ESBL-producing O104:H4 outbreak strain of sequence type (ST) 678 showed this strain to be highly similar to enteroaggregative E. coli (EAEC). Strains of the EAEC pathotype are basically adapted to the human host. To clarify whether in co...

background and objectives: pathogenic strains of escherichia coli are a common cause of acute infectious diarrhea. the aim of this study was to investigate the frequency, virulence markers and antibiotic resistance patterns of diarrheagenic e. coli (dec) isolated from adolescents and adults in hamadan, west of iran. materials and methods: a total of 187 stool samples were collected from adults ...

background and objectives: shiga toxin-producing escherichia coli (stec) have emerged as human pathogens and con- tamination via animal origin has been a major public health concern. we compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of escherichia coli isolated from feces of diarrheatic calves in tehran suburb farms. materials a...

Hemolytic-uremic syndrome, the main cause of acute renal failure in early childhood, is caused primarily by intestinal infections from some Escherichia coli strains that produce Shiga toxins. The toxins released in the gut are targeted to renal endothelium after binding to polymorphonuclear leukocytes. The presence of Shiga toxins in the feces and the circulating neutrophils of 20 children with...

the present work was conducted to compare the occurrence of escherichia coli possessing virulence and esbl genes in backyard and farmed poultry. three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from west bengal, india. among the e. coli isolates of backyard poultry (o2, o10, o25, o55, o60, o106, ut), none of them posse...

Shiga toxin producing Escherichia coli (STEC) are a major cause of food-borne illness worldwide. However, a consensus regarding the role Shiga toxins play in the onset of diarrhea and hemorrhagic colitis (HC) is lacking. One of the obstacles to understanding the role of Shiga toxins to STEC-mediated intestinal pathology is a deficit in small animal models that perfectly mimic human disease. Inf...