A total of 152 clinical isolates of Acinetobacter baumannii from 152 patients were identified by carbon source utilization tests and examined serologically. Polyclonal rabbit immune sera against A. baumannii strains were used in checkerboard tube agglutination tests, and 20 serovars were identified. One (serovar 19) cross-reacted with genospecies 3 (serovar 3), a closely related member of the g...

Using phenotype techniques, characterization was realized of species and serovars of 255 strains of Listeria isolated from human material: 220-86.3% were from patients with possible invasive disease, while 35-13.7% were from colonized healthy individuals. The strains were collected in several regions of Brazil from 1969-2000. In individuals aged 0-10 or 41-60 years old Listeria monocytogenes wa...

Leptospira reactivity in stray and household dogs in Campeche as well as associated risk factors to the seropositivity in household dogs have been herein determined. The survey included 323 dogs, 142 of which were stray dogs and 181 household dogs. Nine Leptospira interrogans serovars were tested by the microagglutination test. Reactivity was 21.3 % (69/323), 17.2 % corresponded to household do...

In this work, IS200 and multi-locus sequence typing (MLST) were used to analyze 19 strains previously serotyped as Salmonella enterica serovar Typhi and isolated in Indonesia (16 strains), Mexico (2 strains), and Switzerland (1 strain). Most of the strains showed the most common Typhi sequence types, ST1 and ST2, and a new Typhi genotype (ST1856) was described. However, one isolate from Mexico ...

Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR...

Chlamydia trachomatis is a pathogen of global public health significance. It is associated with trachoma (serovars A, B, B1 and C), lymphogranuloma venereum (LGV, serovars L1, L2 and L3) and genital infection (serovars D to K). Genital C. trachomatis infection can cause pelvic inflammatory disease (PID) and is a threat to the reproductive health of women in both developed and developing countri...

Ninety two non-penicillinase producing Neisseria gonorrhoeae (non-PPNG) strains were investigated by the restriction enzyme technique with Hind III enzyme. Serogrouping by coenzyme. A with monoclonal antibodies and testing susceptibility to doxycycline were also performed by an agar dilution method. Fifteen different restriction enzyme patterns were shown, of which three represented 50% (46/92)...

By using the monoclonal antibodies methodology we have classified Cuban serovars of leptospires isolated from blood culture of confirmed leptospirosis patients. The serogroups Pomona and Canicola were the most predominant found in our study. Pomona, Mozdok and Canicola serovars were the most prevalent types in these serogroups. Our study has essentially presented a validation of a monoclonal an...

Leptospires are spirochetes that may be free-living saprophytes found in freshwater or may cause acute or chronic infection of animals. The family Leptospiraceae comprises three genera: Leptospira Leptospira Leptonema Leptonema, and Turneriella Turneriella. Within the genus Leptospira, three clades can be distinguished, of pathogens, nonpathogens, and an intermediate group. Leptospires are furt...

Six new serovars of Leptospira interrogans were isolated from opossums (Didelphis marsupialis and Philander opossum) trapped in the Peruvian jungle. The proposed names, type strain designation, and serogroup of the serovars, respectively, were: huallaga, strain M-7, Djasiman serogroup; luis, strain M-6, Tarassovi serogroup; machiguenga, strain MMD-3, Icterohaemorrhagiae serogroup; rioja, strain...