In this paper we present a vectorial Darboux transformation, in terms of ordinary determinants, for the supersymmetric extension of the Korteweg-de Vries equation proposed by Manin and Radul. It is shown how this transformation reduces to the Korteweg-de Vries equation. Soliton type solutions are constructed by dressing the vacuum and we present some relevant plots. On leave of absence from Bei...

PURPOSE The purpose of the study was to determine whether the autodegradation of human βA3-crystallin is due to its intrinsic protease activity. METHODS Recombinant His-tagged human βA3-crystallin was expressed in E. coli and purified by a Ni+2-affinity column chromatographic method. To determine protease activity, the purified crystallin was incubated for 24 h with either sodium deoxycholate...

Serine proteases of the chymotrypsin fold are of great interest because they provide detailed understanding of their enzymatic properties and their proposed role in a number of physiological and pathological processes. We have been developing the macromolecular inhibitor ecotin to be a "fold-specific" inhibitor that is selective for members of the chymotrypsin-fold class of proteases. Inhibitio...

An intracellular serine protease, which is believed to initiate the degradation of several intracellular pyridoxal phosphate-dependent enzymes, was localized by immunofluorescence in atypical mast cells of the lamina propria and in intraepithelial cells of the rat small intestine. Some mucus-secreting goblet cells also contained the protease antigen. Atypical mast cells containing the enzyme we...

The effect of salmon plasma (SP) from Chinook salmon on proteolytic inhibition was investigated. SP was found to inhibit both cysteine and serine proteases as well as protease extracted from Pacific whiting muscle. SP was found to contain a 55kDa cysteine protease inhibitor through SDS-PAGE inhibitor staining. Freeze dried salmon plasma (FSP) and salmon plasma concentrated by ultrafiltration (C...

Proteases are being widely used in various industries like detergent, leather, food and pharmaceuticals.Protease was purified to homogeneity from the seeds of Artocarpus heterophyllus. The enzyme found be a tetramer having molecular mass 74 kDa. Gelatin zymography showed clear band proteolysis. isolated serine protease, as indicated by its inhibition with PMSF. stable at broad pH temperature ra...

The identification of genes with selective expression in specific organs or cell types provides an entry point for understanding biological processes that occur uniquely within a particular tissue. Using a subtraction approach designed to identify genes preferentially expressed in specific tissues, we have identified prostase, a human serine protease with prostate-restricted expression. The pro...

results phylogenetic analysis of the gene revealed that pakistani 1a hcv strains are in the start of third cluster and there is a difference between inter pakistani isolates at primary, secondary and tertiary levels. material and methods patient sera infected with hepatitis c virus, genotype 1a, were obtained from molecular diagnostics lab, cemb, university of the punjab lahore by using bd vacu...

The tarnished plant bug, Lygus hesperus Knight, is a pest that causes considerable economic losses to vegetables, cotton, canola, and alfalfa. Detailed knowledge of its digestive physiology will provide new opportunities for a sustainable pest management approach to control this insect. Little is known about the different protease class contributions to the overall digestion of a specific prote...