Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification of EVs. EVs in bulk were isolated from pooled normal human amniotic fluid (AF) by differential centrifugation followed by IEC or sucrose densi...

The purpose of this work was to improve the separation and yield of pure βand α-trypsin isoforms by ionexchange chromatography and to characterize some physical–chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4C. The sample loading, salt concentration, flow rate and pH of mobile phase we...

The effects of ultrasonic power, treatment time and holding on AZ80 magnesium melt purification by field were studied. results indicate that can accelerate the separation inclusions attain purification. When alloy is treated with power 80 W at 650 °C for 60 s 100 s, best achieved. Moreover, effect degassing AZ91 was also investigated. 150 90 hydrogen content efficiency are 9.6 cm3/100 g 50.5%, ...

Hog kidney Acylase I, an enzyme which hydrolyzes N-acetylL-amino acids, has been purified approximately 40-fold by Greenstein (1). During the course of the purification of hog kidney dialkylfluorophosphatase, it was consistently found that the protein fractions containing DFPasel activity also possessed very high Acylase I activity. Inasmuch as the DFPase and Acylase I activities were found to ...

Purification of peptides is often the most difficult part of the process used to obtain the compounds [1]. Several methods are used for isolation and subsequent purification of the post-synthesis mixture. Depending on the properties of the peptide and the types of impurity, commonly used techniques are ion-exchange chromatography and gel filtration [2,3]. The standard method for isolation is hi...

Interest in new and diverse classes of molecules such as recombinant toxins, enzymes, and blood factors continues to grow for use a biotherapeutics. Compared to monoclonal antibodies, these novel drugs typically lack a commercially available affinity chromatography option, which leads to greater process complexity, longer development timelines, and poor platformability. To date, for both monocl...

We discuss the use of "high-performance" liquid chromatography with electrochemical detection for the quantification fo serotonin (5-hydroxytryptamine) and metabolically related products (i. e., 5-hydroxyindoleacetic acid and 5-hydroxytryptophan) in biological matrices. Two methods are described: one for routine quantification of nanogram amounts or more; the other a two-column purification-sep...

From among a wide variety of protein purification techniques affinity chromatography has proved to be particularly effective for separation of proteolytic enzymes and their inhibitors. In this article, following a general description of affinity adsorbents used for purification of proteinases, we overview a simple separation procedure for some serine proteinases and their inhibitors by way of a...

Restriction enzyme fragmentation and recombinant DNA technology offer powerful tools with which to study gene structure, organization, and expression. Frequently in these studies the need arises to purify on a preparative scale a specific DNA molecule from a mixture. The two most commonly used procedures in such purification are isopycnic centrifugation in dye-salt gradients and gel electrophor...

background: plasminogen is one of the compounds derived from human plasma. activation of plasminogen produces plasmin. plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. the aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine sepharose. materials and methods: normal human plasma was used as the start...