A balance between self-renewal and differentiation of spermatogonial stem cells (SSCs) is required to maintain sperm production throughout male life. The seminiferous epithelium is organized into stages of spermatogenesis based on the complement of germ cell types within a tubular section of the testis. The stages exist in close physical proximity and foster diverse phases of germ cell developm...

As a persistent pollutant, microplastics (MPs) have been reported to induce sperm quantity decrease in mice. However, the related mechanism remains obscure. Therefore, this study is intended explore effects of polystyrene (PS-MPs) on male reproduction and its blood–testis barrier (BTB) impairment. Thirty-two adult Wistar rats were divided randomly into four groups fed with PS-MPs for 90 days at...

Background: An accurate histopathological assessment and reporting of testicular biopsies require an appropriate tissue fixative. We assessed the histological, histochemical, immunohistochemical quality biopsies, comparing 10% formalin versus Bouin solution as fixatives. Methods: This experimental study utilized 20 adult male albino rats equally divided into five cages for 30 days. By end exper...

background: quantum dots (qds) are new types of fluorescent materials for biological labeling. qds toxicity study is an essential requirement for future clinical applications. therefore, this study aimed to evaluate cytotoxic effects of cdse: zns qds on male reproductive system. materials and methods: in this experimental study, the different concentrations of cdse: zns qds (10, 20 and 40 mg/kg...

To assess the regulation of stem factor factor (SCF) gene expression during spermatogenesis, we tested the effects of hormones (FSH, testosterone, and 17beta-estradiol) and some growth factors [transforming growth factor-beta (TGF beta), TGF alpha, tumor necrosis factor-alpha, and activin] on SCF gene expression by using a transillumination-assisted microdisection technique, a seminiferous tubu...

2,5-Hexanedione (2,5-HD), a taxol-like promoter of microtubule assembly, and carbendazim (CBZ), a colchicine-like inhibitor of microtubule assembly, are two environmental testicular toxicants that target and disrupt microtubule function in Sertoli cells. At the molecular level, these two toxicants have opposite effects on microtubule assembly, yet they share the common physiologic effect of inh...

INTRODUCTION Ghrelin is a hormone which has effects on the secretion of growth hormone, gastrointestinal system, cardiovascular system, cell proliferation and reproductive system. The present study we focused on the relation between ghrelin and GHS-R1a gene expression and the regulation of their expression in the testes of diabetic rats. MATERIAL AND METHODS 40 male Wistar albino rats were di...