In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

target modification and reduced drug accumulation are the main resistance mechanisms against fluoroquinolone antibiotics in pseudomonas aeruginosa. we performed a genotypic characterization of three major mex multidrug efflux pumps (mexab-oprm, mexxy-oprm and mexcd-oprj) in ciprofloxacin resistant clinical isolates of p. aeru­ginosa, collected from tehran, iran this was followed by sequencing a...

Quantitative reverse transcription PCR (RT-PCR) is typically used to assay transcript abundance (often generalized as “gene expression”) by measuring a specific cDNA level. The method is very sensitive and is suitable for a broad range of cDNA concentrations. Its reliability depends on, among other factors, appropriate normalization (for a review, see References 1 and 2). The preferred method o...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 6 751–762 www.jcb.org/cgi/doi/10.1083/jcb.201312062 JCB 751 Correspondence to Kota Saito: [email protected] Abbreviations used in this paper: BFA, Brefeldin A; CT, C terminus; ERGIC, ER– Golgi intermediate compartment; GEF, guanine-nucleotide exchange factor; MBP, maltose-binding protein; qRT-PCR, quantitative RT-PCR; R...

PURPOSE Molecular analysis of melanoma sentinel nodes (SN) is sensitive, but poorly specific because metastases cannot be distinguished from benign nevus inclusions (BNI). We investigated whether quantitative reverse transcription-PCR (RT-PCR) detection of MART-1 and tyrosinase mRNAs could improve this specificity and contribute to SN assessment. EXPERIMENTAL DESIGN Two hundred twenty SNs fro...