AIMS In this study we report our results with storage of cryopreserved semen intended for preservation and subsequent infertility treatment in men with testicular cancer during the last 18 years. METHODS Cryopreserved semen of 523 men with testicular cancer was collected between October 1995 and the end of December 2012. Semen of 34 men (6.5%) was used for fertilization of their partners. The...

It is now possible to store turkey semen for 4 to 24 h without appreciable loss in fertility and hatchability. Optimum conditions for 24 or 48 h storage have not been fully determined. Development of new storage media, allowing the insemination of very low numbers of spermatozoa, may provide interesting possibilities regarding the use of superior sires. This study was conducted to evaluate effe...

The successful application of assisted reproductive techniques (ARTs) in ovine as other mammal species relies on many factors among which the quality semen used. After collection, samples are generally processed for storage (liquid or cryoconservation) before being used insemination vitro embryo production. During liquid process, sperm cells exposed to artificial conditions lead oxidative stres...

In order to ascertain the microbiological quality of stored semen specimens processed for artificial insemination by a donor (AID), we developed a PCR assay targeting the chlamydial plasmid to detect Chlamydia trachomatis in semen. The lower limit of detection of this assay corresponded to 2.5 to 5 elementary bodies per microl of semen. A total of 669 cryopreserved ejaculates from 97 asymptomat...

The objective of this experiment was to test a hypothesis that L-carnitine supplemented diet would improve semen characteristics in 6 adult crossbred AI boars (Hampshire × Pietrain). The control and the tested diet were identical except the tested period (8 weeks) when the diet was supplemented with 2 g of l-carnitine per boar per day. The semen was collected regularly weekly by a gloved-hand t...

The aim of the present study was to determine the effects of green tea polyphenol on the quality of canine semen after long-term storage at 5°C. The supplementation of a Tris-egg yolk extender with polyphenol (0.5, 0.75, or 1mg/mL) increased the motility and viability of sperm preserved for four weeks at 5°C.

We have approached the problem of refrigerated storage of epididymal sperm samples from red deer by comparing three options: storing the genital (testicles within the scrotum), diluting the semen in extender or diluting the semen in extender supplemented with an anti-oxidant. Twenty-nine pairs of testes were collected. Spermatozoa from one of each of the pairs were immediately recovered, and di...

Density gradient centrifugation of semen is commonly used in many assisted reproduction techniques. Although gradients have the potential to isolate and enrich motile and viable spermatozoa, the centrifugation force presents a stress factor to cell organelles and membranes. The objective of the study was to evaluate the impact of density gradient centrifugation stress on sperm capacitation dyna...