AIM To evaluate different thawing temperatures and duration on the post-thaw semen quality of Indian yaks bulls. MATERIALS AND METHODS Semen ejaculates from four different yak bulls were collected using artificial vagina method and extended with tris extender containing 6.4% glycerol at 35°C, cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at 4-5°C for 4 h and frozen in French...

The current study was performed to test the of quality as well as the DNA integrity of preserved liquid and frozen buffalo spermatozoa due to addition of different concentrations of glutathione (0.50, 1.00, 2.00 and 3.00 mM). Liquid semen was preserved for 120 hour with or without addition of glutathione and examined for progressive motility, viability, plasma and acrosomal membranes integrity ...

Aim: The present study was carried out to examine the relationships among frozen-thawed semen fertility, physical parameters, seminal quality, and testosterone concentration in Murrah buffalo bulls. Materials and Methods: A total of 30 breeding Murrah buffalo bulls (either progeny tested or under progeny testing program) were randomly selected from two government bull farms in Punjab. None of t...

Very low levels of somatic mitochondrial (mt)DNA deletions have been identified in the semen of infertile men. It has been suggested that these mutations cause infertility through an effect on sperm motility, but there has been no direct evidence to show that mutant mtDNA can affect sperm function. We have carried out semen analysis on a male harbouring the A3243G mtDNA mutation and show that h...

Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen sam...

This study was conducted at the National Artificial Insemination Center, Ethiopia with an objective of evaluating breeding soundness, effect of age and breed on semen quality and fertility of bulls. The evaluation involved 45bulls (36 breeding and 11 pre-service young bulls) consisting of Holstein Friesian, Jersey, Borana and Cross breeds (Borana X Holstein Frisian). Semen was collected using a...

A study was conducted to evaluate the effects of dietary lipid sources on the reproductive performances of Hy-line Silver-Brown cockerels. Five different lipid sources namely fish oil, sunflower oil, high oleic acid sunflower oil, tallow and an equal (50:50) proportion of linseed and fish oil (representing the control diet), were used in formulating five isoenergetic and isonitrogenous diets (3...

The present study was conducted to test the post-thaw sperm characteristics (motility, viability, acrosomal and plasma membranes integrity) through addition of different concentrations of BSA (0.50, 1.00, 5.00, 10.00 and15.00 %) to semen diluents lacking egg yolk. Semen was collected from four mature buffalo bulls, twice weekly using artificial vagina. Sperm characteristics (motility, viability...

This study was aimed to evaluate the effects of dietary n-6:n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6:n-3 ratios (14.4 and 6.6) and two Vitamin E levels (200 and 400 mg kg-1 ). During 16 weeks of treatment, fresh semen was collected at weeks 0, 8, 1...

Objective: Using correlation analysis between the sperm spontaneous acrosome reaction rate (SARR) and routine semen parameters in male infertility patients, we explored the value of the sperm spontaneous acrosome reaction in the evaluation of sperm functions. Methods: The participants comprised of 219 male infertility patients who had visited the Center for Reproductive Medicine, Shandong Unive...