Background: Artificial insemination is a valuable tool in genetic improvement programs and widely used breeding technique in farm animals. Semen processing and cryopreservation causes considerable damage to sperm DNA, motility apparatus, plasma membrane, acrosomal cap and thus, reduced fertility. Selenium is an integral part of glutathione peroxidase (GSH-Px), an enzyme which protects cell inte...

Origanum vulgare contains high levels of phenolic compounds such as gallic acid and polyphenols such as rosmarinic acid and quercetin. The purpose of this study was to investigate the effects of Origanum vulgare extract, as a natural antioxidant, on freezing-thawing semen quality in Holstein bulls. Three Holstein bulls (5-6 years old, mean live weight 800 kg) were used for semen collection twic...

Antibiotics are mandatory additives in semen extenders to control bacterial contamination. The worldwide increase in resistance to conventional antibiotics requires the search for alternatives not only for animal artificial insemination industries, but also for veterinary and human medicine. Cationic antimicrobial peptides are of interest as a novel class of antimicrobial additives for boar sem...

This study assessed the effects of common extenders and diluents on the spermatozoa of African catfish (Clarias gariepinus), with the intent of obtaining a semen preservation protocol that can serve as a means of making fingerlings available to fish farmers all year round. Semen samples (milt) were pooled from mature broodstock males and pre-extension qualities were evaluated. Egg yolk, tomato ...

Three experiments evaluated the effects of dietary Se and vitamin E on the ultrastructure of spermatozoa, ATP concentration of spermatozoa, and the effects of adding sodium selenite to semen extenders on subsequent sperm motility. The experiment was a 2 x 2 arrangement of treatments in a randomized complete block design. A total of 10 mature boars were fed from weaning to 18 mo of age diets for...

In the present study the Authors developed a new modified boar semen extender for short-term liquid storage, based on the use of amikacin sulphate and fructose rather than gentamicin and glucose. The new extender (ME-S) was evaluated and compared in vitro to commercial ones (CRONOS, TRIXcell) and to a modified extender designated for long term storage (ME-L) for progressive motility. Progressiv...

AIM The aim was to compare commercially available soybean milk-based extenders, viz. Bioxcell(®) and Optixcell(®) (IMV, France) with standard Tris-citrate-fructose-egg yolk-glycerol (TFYG) extender for cryopreservation of buffalo semen. MATERIALS AND METHODS Semen was collected twice a week in artificial vagina from six sexually mature, 4-6 years old, healthy breeding bulls of Surti buffalo b...

This study was conducted to evaluate rabbit semen characterization before freezing and after thawing. Also, to determine the sex ratio of rabbit offspring’s after artificial insemination (AI) using semen selected by Percoll or swim up method. Semen was collected from New Zealand white rabbit bucks (n=9) and 45 rabbit does in three groups (15 in each) were inseminated with frozen semen (control,...