tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. Cunha-Filho, in his commentary on the practice recommendations by Agarwal et al. (1), described the current status of sperm DNA fragmentation (SDF) testing. He discussed the pitfalls of the current practice in using intracytoplasmic sperm injection (ICSI) in bypassing male factors on one hand, while elaborating on...

AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

Diagnosis of sperm DNA integrity of semen sample is important for consistently high reproductive efficiency. The conventional parameters of semen analysis take into account morphology, motility, and concentration of spermatozoa in the sample, which are insufficient for evaluation of reproductive potential. Current studies have implicated abnormal organization of genomic material in sperms as a ...

background: it has been shown that reactive oxygen species (ros) can lead to deleterious effects on a range of sperm parameters. vitamins e and c are capable of reducing ros levels and lipid peroxidation. objective: the aim of study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) and vitamins e and c in sperm and seminal plasma of asthenoteratozoospermic a...

A semen sample from a stallion infected during the 2010 equine arteritis virus (EAV) outbreak was received for viral isolation prior to castration of the animal. The virus was identified using a polyclonal antibody immunofluorescence test. Reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify a region of the GP5 gene with primers GL105F and GL673R. The PCR products were p...

evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear dna integrity of spermatozoa. it has been suggested that altered nuclear chromatin struc...

introduction: the diagnostic work-up of allergic rhinitis (ar) is first and foremost based on the combination of clinical history data and results of skin prick tests (spt). other tests, including specific ige measurement, nasal challenge, and, as a third option, component resolved diagnosis or basophil activation test, may be useful when the diagnosis is difficult because of polysensitization ...

Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...