In pharmaceutical drug discovery and agricultural crop product discovery, in vivo bioassay experiments are used to identify promising compounds for further research. The reproducibility and accuracy of the bioassay is crucial to be able to correctly distinguish between active and inactive compounds. In the case of agricultural product discovery, a replicated dose-response of commercial crop pro...

Currently, the only accepted assay with which to detect active Clostridium botulinum neurotoxin is an in vivo mouse bioassay. The mouse bioassay is sensitive and robust and does not require specialized equipment. However, the mouse bioassay is slow and not practical in many settings, and it results in the death of animals. Here, we describe an in vitro cleavage assay for SNAP-25 (synaptosome-as...

Establishment of the nitrogen-fixing nodulation symbiosis between legumes and rhizobia requires plant-wide reprogramming to allow infection and development of nodules. Nodulation is regulated principally via a mechanism called autoregulation of nodulation (AON). AON is dependent on shoot and root factors and is maintained by the nodulation autoregulation receptor kinase (NARK) in soybean. We de...

This paper reports the development, characterization, and application of a separation-free, homogeneous, and simple to perform fluorescence polarization bioassay for paclitaxel. The bioassay is based on the binding interaction of paclitaxel to tubulin, the receptor protein on which this drug acts. The bioassay was carried out in a competitive format where the paclitaxel and a synthetic fluoresc...

PubChem's BioAssay database (https://pubchem.ncbi.nlm.nih.gov) has served as a public repository for small-molecule and RNAi screening data since 2004 providing open access of its data content to the community. PubChem accepts data submission from worldwide researchers at academia, industry and government agencies. PubChem also collaborates with other chemical biology database stakeholders with...

A laboratory bioassay was developed for testing tarnished plant bug populations for resistance development to the neonicotinoid insecticides imidacloprid and thiamethoxam. The bioassay allows for the determination of LC50 values by feeding known doses of the insecticides to adult tarnished plant bugs in a honey-water solution. Field populations of plant bugs from several locations in the mid-So...

-Peripheral-blood basophils were counted, in thick smears, in samples from patients with primary bronchial carcinoma, from patients in the same wards and from normal individuals. The median counts for patients with other chest conditions (15-5/4d) and bronchial-carcinoma patients free of tumour months to years after resection (16/1l) did not differ from those for normal individuals (19/1,u), bu...

BACKGROUND Experimental investigation of stroke, in particular the evaluation of therapeutic maneuvers, is difficult because even well-controlled experiments show considerable variability. Optimal use of resources requires efficient statistical analysis. SUMMARY OF REVIEW A body of experience in pharmacology that is directly applicable to stroke studies is reviewed. The general approach, thou...

Fagopyrum esculentum Moench (buckwheat) is a dicot species from the Polygonaceae family used as cover crop in agricultural systems featured with remarkable allelopathic potential for weed control, helping herbicide-resistance management and promoting substantial reductions herbicide applications. The aim of this research was to examine aqueous extracts seeds aerial part buckwheat on seed germin...

an agar well diffusion bioassay method for determination of clarithromycin in human plasma, using micrococcus luteus atcc 9341 as the assay organism, was compared with a selective high-performance liquid chromatographic (hplc) method with uv detection. spiked plasma was used to prepare standard and control samples for both methods. the results of the bioassay analyses with spiked plasma samples...