Electron microscopes have been widely used to investigate cell structures under high resolution. A scanning electron microscope (SEM) provides tridimensional images of a cell surface, while a transmission electron microscope (TEM) is commonly employed to observe the internal structure changes of host cells after bacterial adherence and invasion. The SEM and TEM can reveal bacterial location, bi...

Introduction “Second best no more” was the title of an article written by David C. Joy for Nature Materials [1]. The article was for highlighting a breakthrough made by a team formed between Brookhaven National Laboratory (BNL, USA) and Hitachi High Technologies Corporation (HHT, Japan). Sub-angstrom secondary electron (SE) images were obtained on a Hitachi HD-2700, which is a combined scanning...

it is only thirteen years since the scanning electron microscope has been available commercially. yet, even in this short period of time, this instrument has been a powerful tool in the investigation of topography, electrical and magnetic properties, crystal structure, cathodoluminescent characteristics etc. of solid specimens. today, this type of microscope has opened its place alongside the c...

Spin-polarized scanning electron microscopy (spin SEM) is a method for observing magnetic-domain structures by detecting the spin polarization of secondary electrons. It has several unique abilities such as detection of full magnetization orientation and high-spatial-resolution measurement. Several spin-SEM experiments have demonstrated that it is a promising method for studying various types o...

Once trained, the human brain is an excellent image processor; to replicate that skill for scanning electron microscope (SEM) image assessment demands considerable computing power. We have evaluated parallel computing for this purpose and developed an image processing tool for the automatic optimisation of electron gun alignment. The instrumental setup for this work was a Carl Zeiss 1430VP SEM ...

The hydride distribution in a cracked Zircaloy cladding tube has been investigated by neutron radiography, Scanning Electron Microscopy (SEM) and Hydrogen measurements. The crack occurred during handling in the spent fuel pool. Back Scattered Electron images showed some unexpected contrasts in zones with high hydrogen concentrations near cracks. This phase has been studied by SEM and EPMA. Afte...

A series of copper-based systems containing two different nanocomposites (Cu2O/CuO and Cu2O/Cu) was synthesized by the egg white assisted auto-combustion route. This method distinguished simplicity its steps, low cost, one-pot synthesis process at temperature and, short time. The characterization as prepared carried out using X-ray diffraction (XRD), Fourier-transform infrared (FTIR), Scanning ...

The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at...

We have used focused ion beam (FIB) milling and scanning electron microscopy (SEM) to prepare samples of known thickness for measurement of the electron scattering elastic and inelastic mean free paths (EMFP and IMFP respectively) of three metallic glasses, Cu(64.5)Zr(35.5), Zr(50)Cu(45)Al(5), and Al(87)Y(7)Fe(5)Cu. After measuring the EMFP and IMFP in a scanning transmission electron microscop...

caco3 nanoparticles have been synthesized via heat-treatment of a new precursor and solid state reaction. effect of calcinations temperature and quantity of surfactant on particle size has been investigated. the products were characterized by x-ray diffraction (xrd), scanning electron microscopy (sem), transmission electron microscopy (tem) and fourier transform infrared (ft–ir) spectroscopy.