The effect of interleukin-1 alpha (IL-1 alpha) and murine epidermal growth factor (EGF) on incorporation of endogenously produced collagenase in the extracellular matrix of soft connective tissue was studied in an in vitro model system using periosteal explants obtained from rabbit calvariae. Immunohistochemical analysis indicated the highest level of collagenase in explants cultured for 72 hou...

objective(s):p in vitro chemosensitivity and resistance assays (csras) are a promising tool for personalized treatment of glioblastoma multiform (gbm). these assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. we aimed to assess the feasibility and validity of utilizing stereotactic biopsies of gbm in csras. materials and methods: single...

Use of light-emitting diodes (LEDs) in strawberry vitro multiplication The refinement plant tissue culture techniques is great importance for optimizing rates and improving the quality micropropagated changes, either or latest models light sources light..The objective this work was to evaluate effect type on Tudla cultivar. Strawberry sprouts were grown 30 days MS medium plus 100 mg L-1 myo-ino...

PURPOSE Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for c...

To screen for suitable conditions cluster bud induction of Cremastra appendiculata . The aseptic germination protocorms of C.  seeds were used as explant materials to explore the effects different basic media, types plant growth regulators, anti-browning agents and their concentrations on buds of results showed that: 1/4MS medium was buds growing; when concentratio...

Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental medicinal and aromatic plants. Therefore, optimization of techniques is very important. In this study, the effects different Murashige Skoog (MS) salt levels (25%-200%) on in vitro Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different MS ...

four separate experiments were conducted to study the effects of factors controlling the establishment of explants from two walnut genotypes z60 and z63 (damavand). factors were explant type, sampling time, explant orientation on the growth medium and antioxidants type. the explants were collected from the collection garden of tooyserkan walnuts research station. four explant types (apical meri...